Method for producing platelets from megakaryocytes
View Patent ↗The present invention relates to a method for producing platelets from mature megakaryocytes. More particularly, the invention relates to an ex vivo method for producing platelets, from mature megakaryocytes, said method comprising a step of subjecting a suspension of mature megakaryocytes to a flow having a minimal shear rate of 600 s −1 on a solid phase coated with Von Willebrand factor.
1. An ex vivo method for producing platelets, comprising a step of subjecting a suspension of cells comprising mature megakaryocytes to a flow permitting exposure to a shear rate sufficient for platelet generation, on a solid phase coated with Von Willebrand factor (VWF) or a fragment or variant thereof which binds to GPIb thereby forming platelets.
2. A method according to claim 1 , wherein said fragment or variant of VWF is selected from the group consisting of the 52/48-kDa tryptic fragment of VWF; Staphylococcus aureus V-8 protease-digested VWF; VWF concentrates for therapeutic use and VWF mutants responsible for type 2N von Willebrand disease.
3. The method according to claim 1 , wherein said mature megakaryocytes are exposed to a shear rate of at least 1000 s−1.
4. The method according to claim 1 , wherein said cell concentration in the suspension is between 0.5 and 4×10 6 /mL.
5. The method according to claim 1 , wherein said cell concentration in the suspension is at least 4×10 6 /mL.