Polynucleotides for expression of microbial starch branching enzymes in plants for production of plants with improved yield
Recombinant constructs and methods useful for improvement of plants are provided. In particular, recombinant constructs comprising promoters functional in plant cells positioned for expression of polynucleotides encoding starch branching enzymes from microbial sources are provided. The disclosed constructs and methods find use in production of transgenic plants to provide plants, particularly crop plants, having improved yield.
1. A recombinant DNA construct comprising a promoter functional in a plant cell, wherein said promoter is positioned to provide for expression of a polynucleotide encoding a polypeptide, and wherein said polynucleotide is selected from the group consisting of:
(a) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 25407;
(b) a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 1720;
(c) a polynucleotide having at least 90% sequence identity to the polynucleotide of (a) or (b), and encoding a functional homolog of said polypeptide having the amino acid sequence of SEQ ID NO: 1720; and
(d) a polynucleotide encoding a polypeptide having at least 90% sequence identity to a polypeptide having the amino acid sequence of SEQ ID NO: 1720, wherein said encoded polypeptide is a functional homolog of said polypeptide having the amino acid sequence of SEQ ID NO: 1720, wherein expression of said recombinant DNA construct increases yield.
2. A transgenic plant comprising a recombinant DNA construct, wherein said construct comprises a promoter functional in a plant cell, wherein said promoter is positioned to provide for expression of a polynucleotide encoding a polypeptide, and wherein said polynucleotide is selected from the group consisting of:
(a) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 25407;
(b) a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 1720;
(c) a polynucleotide having at least 90% sequence identity to the polynucleotide of (a) or (b), and encoding a functional homolog of said polypeptide having the amino acid sequence of SEQ ID NO: 1720; and
(d) a polynucleotide encoding a polypeptide having at least 90% sequence identity to a polypeptide having the amino acid sequence of SEQ ID NO: 1720, wherein said encoded polypeptide is a functional homolog of said polypeptide having the amino acid sequence of SEQ ID NO: 1720, wherein expression of said recombinant DNA construct increases yield.
3. The transgenic plant according to claim 2 , wherein said plant is a crop plant.
4. The transgenic plant according to claim 3 , wherein said plant is maize.
5. The transgenic plant according to claim 3 , wherein said plant is soybean.
6. The transgenic plant according to claim 2 , wherein said plant is soybean, cotton, canola, wheat, sunflower, sorghum, alfalfa, barley, millet, rice, tobacco, a fruit, a vegetable, or turf grass plant.
7. The transgenic plant according to claim 2 , wherein said polynucleotide having at least 90% sequence identity to the polynucleotide of (a) or (b) has at least about 95% sequence identity with SEQ ID NO: 25407.
8. The transgenic plant according to claim 2 , wherein said polynucleotide having at least 90% sequence identity to the polynucleotide of (a) or (b) has at least about 98% sequence identity with SEQ ID NO: 25407.
9. The transgenic plant according to claim 2 , wherein said polynucleotide is a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 1720.
10. The recombinant DNA construct according to claim 1 , wherein said polynucleotide is a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO: 1720.
11. The transgenic plant according to claim 2 , wherein said encoded polypeptide has at least 95% sequence identity to SEQ ID NO: 1720.
12. The transgenic plant according to claim 2 , wherein said functional homolog provides 1,4-alpha-glucan branching enzymatic activity.
13. The transgenic plant according to claim 2 , wherein said functional homolog comprises one or more conservative amino acid substitutions in SEQ ID NO: 1720.
14. The transgenic plant according to claim 2 , wherein said promoter comprises a tissue specific enhancer.
15. The transgenic plant according to claim 2 , wherein said polynucleotide further encodes a transit peptide for targeting to a plastid.