Detection of oligosaccharides
Provided herein are processes for detecting oligosaccharides in a biological sample. In specific instances, the biological sample is provided from an individual suffering from a disorder associated with abnormal glycosaminoglycan accumulation.
1. A composition comprising an isolated oligosaccharide of the following formula: GlcNS-IdoA2S-GlcNS.
2. The composition of claim 1 , wherein the oligosaccharide present in the composition is less than 70% by weight non-reducing end unsaturated oligosaccharide.
3. The composition of claim 1 , wherein the oligosaccharide present in the composition is less than 50% by weight non-reducing end unsaturated oligosaccharide.
4. The composition of claim 1 , further comprising chromatographic resin.
5. A composition comprising of claim 1 , wherein the oligosaccharide further comprises a detectable label attached to the reducing end thereof, wherein the detectable label is 2 H-2-anthranilic acid, 2 H-2-aminopyridine, 2 H-methyl iodide, 13 C methyl iodide, 2 H-pyridyl amine, 2 H-biotin, an antibody, an affinity label, a radiolabel, a chromophore, or a fluorescent label.