Microorganisms for producing butadiene and methods related thereto

1. A non-naturally occurring microbial organism, said microbial organism having a butadiene pathway and comprising at least two exogenous nucleic acids each encoding a butadiene pathway enzyme expressed in a sufficient amount to produce butadiene, wherein said butadiene pathway comprises the butadiene pathway enzymes of an acetyl-CoA:acetyl-CoA acyltransferase, an acetoacetyl-CoA reductase, a 3-hydroxybutyryl-CoA dehydratase, a crotonyl-CoA reductase (aldehyde forming), a crotonaldehyde reductase (alcohol forming), a crotyl alcohol kinase, a 2-butenyl-4-phosphate kinase and a butadiene synthase; said microbial organism further comprising:

(a) a reductive TCA pathway comprising at least one exogenous nucleic acid encoding a citryl-CoA synthetase or a citryl-CoA lyase; or

(b) a reductive TCA pathway comprising at least one exogenous nucleic acid encoding a phosphoenolpyruvate carboxylase or a phosphoenolpyruvate carboxykinase.

2. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprising (a) further comprises an exogenous nucleic acid encoding an enzyme selected from an ATP-citrate lyase, a citrate lyase, a fumarate reductase, an alpha-ketoglutarate:ferredoxin oxidoreductase, a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin oxidoreductase, ferredoxin, and combinations thereof.

3. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprising (b) further comprises an exogenous nucleic acid encoding an enzyme selected from an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, and combinations thereof.

4. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises two, three, four, five, six or seven exogenous nucleic acids each encoding an enzyme of the butadiene pathway.

5. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises exogenous nucleic acids encoding each of the enzymes of an acetyl-CoA:acetyl-CoA acyltransferase, an acetoacetyl-CoA reductase, a 3-hydroxybutyryl-CoA dehydratase, a crotonyl-CoA reductase (aldehyde forming), a crotonaldehyde reductase (alcohol forming), a crotyl alcohol kinase, a 2-butenyl-4-phosphate kinase and a butadiene synthase.

6. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises two or three exogenous nucleic acids each encoding enzymes of (a) or (b).

7. The non-naturally occurring microbial organism of claim 6 , wherein said microbial organism comprising (a) comprises four exogenous nucleic acids encoding a citryl-CoA synthetase, a citryl-CoA lyase, a fumarate reductase, and an alpha-ketoglutarate: ferredoxin oxidoreductase;

wherein said microbial organism comprising (b) comprises four exogenous nucleic acids encoding a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase or a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H 2 hydrogenase; or

wherein said microbial organism comprising (a) or (b) comprises two exogenous nucleic acids encoding CO dehydrogenase and H 2 hydrogenase.

8. The non-naturally occurring microbial organism of claim 1 , wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

9. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

10. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises two exogenous nucleic acids each encoding an enzyme of the butadiene pathway.

11. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises three exogenous nucleic acids each encoding an enzyme of the butadiene pathway.

12. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises four exogenous nucleic acids each encoding an enzyme of the butadiene pathway.

13. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises five exogenous nucleic acids each encoding an enzyme of the butadiene pathway.

14. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises six exogenous nucleic acids each encoding an enzyme of the butadiene pathway.

15. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises seven exogenous nucleic acids each encoding an enzyme of the butadiene pathway.

16. A method for producing butadiene, comprising culturing the non-naturally occurring microbial organism of claim 1 under conditions and for a sufficient period of time to produce butadiene.