Biological detector and method
View Patent ↗A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.
1. A method for detecting a particle/target analyte in a sample, the method comprising:
providing a device comprising a magnet configured to produce a magnetic field of less than or equal to 1.5 T, a vessel configured to hold a sample and being located with the magnetic field, and a radio frequency (RF) coil located proximate the magnetic field;
introducing magnetic particles comprising target-specific moieties to a sample comprising a target analyte and non-target species, wherein the sample and the magnetic particles are within the vessel; and
non-optically detecting at least one particle/target analyte complex in the sample in the vessel by activating the RF coil proximate the magnetic field to provide energy at a frequency sufficient for nuclear magnetic resonance detection of the at least one particle/target analyte complex in the vessel.
2. The method according to claim 1 , wherein the detecting step is performed in the presence of the non-target species in the sample.
3. The method according to claim 2 , wherein the nuclear magnetic resonance detection comprises detecting a signal due to a change in relaxation time.
4. The method according to claim 3 , wherein the relaxation rate changes in the presence of the particle/target analyte complexes.
5. The method of claims 4 , wherein said changes are selected from relaxation times T 1 , T 2 , T 2 *, or a combination thereof.
6. The method according to claim 1 , wherein the RF coil wraps around the vessel.
7. The method according to claim 1 , wherein the magnet is a permanent magnet.
8. The method according to claim 1 , wherein the target-specific moieties are antibodies.
9. The method according to claim 8 , wherein the biological sample is blood or urine.
10. The method according to claim 1 , wherein the sample is a biological sample.
11. The method according to claim 1 , wherein the magnetic particles are superparamagnetic particles.