Device for increasing the depth discrimination of optical imaging systems
A device for increasing the depth discrimination of optical imaging systems includes elements for structuring illumination light in an illumination beam of the optical imaging system by application of a grating-like pattern in a conjugate object plane of the optical imaging system. The device further elements for varying the position of the grating-like patterns along an optical axis of the illuminating beam, and elements that vary the position of an image of the grating-like pattern on a specimen in an object plane. The invention may include a grating changer connected to an electronic control system or to a control program of the optical imaging system for automatically changing the grating-like pattern to at least one further grating-like pattern and/or for removing the grating like pattern from the illuminating beam path.
1. A device for increasing the depth discrimination of optical imaging systems, comprising:
means for structuring illumination light in an illumination beam of the optical imaging system, including a grating-like pattern in a conjugated object plane of the optical imaging system;
means for varying the position of the grating-like pattern along an optical axis of the illumination beam;
means for varying the lateral position of an image of the grating-like pattern on a specimen in an object plane;
an electronic control system; and
a grating changer connected with the electronic control system, wherein the grating changer is arranged to automatically change the grating-like pattern to a different grating-like pattern or remove the grating-like pattern from a path of the illumination beam.
2. The device of claim 1 , wherein the grating changer comprises a transparent substrate which can be moved relative to the optical axis of the illuminating beam by motor action and on which a plurality of grating-like patterns differing from each other are provided.
3. The device of claim 1 , wherein the grating changer comprises several substrates, each with one grating-like pattern, which can be placed in the illuminating beam path by motor action.
4. The device of claim 1 , wherein the grating changer comprises a spatial light modulation unit for generating varied grating-like patterns.
5. The device of claim 1 , wherein the grating changer is coupled with an objective changer of the optical imaging system via the electronic control system and the electronic control system selects the grating-like pattern to be brought into the illumination beam path as a function of the magnification and/or numerical aperture of the objective selected.
6. An optical imaging system including a device for increasing the depth discrimination, the device comprising:
means for structuring illumination light in an illumination beam, including a grating-like pattern in a conjugated object plane;
means for varying the position of the grating-like pattern along an optical axis of the illumination beam;
means for varying the lateral position of an image of the grating-like pattern on a specimen in an object plane;
an electronic control system; and
a grating changer connected with the electronic control system, wherein the grating changer is arranged to automatically change the grating-like pattern to a different grating-like pattern or remove the grating-like pattern from the illumination beam path.
7. The optical imaging system of claim 6 , wherein the optical imaging system is a microscope.
8. The optical imaging system of claim 6 , wherein the grating changer comprises a transparent substrate which can be moved relative to the optical axis of the illuminating beam by motor action and on which a plurality of grating-like patterns differing from each other are provided.
9. The optical imaging system of claim 6 , wherein the grating changer comprises several substrates, each with one grating-like pattern, which can be placed in the illuminating beam path by motor action.
10. The optical imaging system of claim 6 , wherein the grating changer comprises a spatial light modulation unit for generating varied grating-like patterns.
11. The optical imaging system of claim 6 , wherein the grating changer is coupled with an objective changer of the optical imaging system via the electronic control system, and the electronic control system selects the grating-like pattern to be brought into the beam path as a function of the magnification and/or numerical aperture of the objective selected.
12. The optical imaging system of claim 6 , wherein, when a rest position is set on the device or when the device is removed from the optical imaging system, the grating changer removes the grating-like pattern from the illumination beam.
13. The optical imaging system of claim 6 , wherein when a working position is set on the device or when the device is inserted into the optical imaging system, the grating changer inserts the grating-like pattern into the illumination beam.