Decellularization and recellularization of donor tissues for minimized or obviated rejection reactions
The method of tissue transplantation involving recellularizing of a donor organ with the utilization of the recipient's cytokines collected from the recipient's blood plasma at less than systemic pressure, and at the temperature greater than freezing and less than normal systemic temperature of the recipient's blood. Specifically, a method of harvesting a platelet-derived growth factors from platelet rich plasma (PRP), the growth factors having increased weight.
1. A method for adapting tissue for trans-organism implantation comprising the steps of:
selecting donor tissue from a first organism;
decellularizing said donor tissue;
selecting stem cells and cytokines from a second organism, said cytokines being extracted from said second organism's blood, at least in part when such blood is at less than systemic pressure; and
infusing said donor tissue with said stem cells and said cytokines.
2. The method of claim 1 wherein said donor tissue is an organ.
3. The method of claim 2 wherein said donor tissue is a kidney.
4. The method of claim 1 wherein said cytokines being extracted is performed when said blood of said second organism is at a pressure less than ambient pressure.
5. The method of claim 1 wherein said cytokines being extracted is performed when said blood of said second organism is at a temperature greater than freezing and less than normal systemic temperature of said second organism.
6. The method of claim 1 further comprising the step of implanting said donor tissue, after said infusing, into said second organism.
7. The method of claim 6 , further comprising a step of infusing said donor tissue with a second measure of said stem cells and a second measure of said cytokines, after said implanting.
8. The method of claim 6 , further comprising the step of infusing said donor tissue with autologous plasma, after said implanting.
9. The method of claim 1 , wherein said first organism and said second organism are different species.
10. The method of claim 9 wherein said donor tissue is an organ.
11. The method of claim 10 wherein said donor tissue is a kidney.
12. Modified tissue for trans-organism implantation by the method of any one of claim 1 , 6 , 4 , 5 , 7 , or 8 .
13. The modified tissue of claim 12 wherein said donor tissue is an organ.
14. The modified tissue of claim 13 wherein said donor tissue is a kidney.
15. The Modified tissue of claim 12 wherein said donor tissue is from a first species and said cells and cytokines are from a second species.
16. The modified tissue of claim 15 wherein said donor tissue is an organ.
17. The modified tissue of claim 16 wherein said donor tissue is a kidney.