Method of preparing rosemary nanoparticles and encapsulated rosemary nanoparticles
A method of preparing rosemary nanoparticles includes providing a solution including rosemary extract, spraying the rosemary extract solution into boiling water under ultrasonic conditions to produce a sonicated mixture, and freeze-drying the sonicated mixture to produce rosemary nanoparticles. The rosemary nanoparticles and encapsulated rosemary nanoparticles with polymers can have a particle size of about 69 nm to about 99 nm. The rosemary nanoparticles and encapsulated rosemary nanoparticles with polymers can be prepared without the use of metals.
1. A method of preparing rosemary nanoparticles, comprising the steps:
(a) combining about 30 mL dichloromethane (DCM) and about 150 mg rosemary extract to produce a first solution,
(b) adding the first solution to an aqueous second solution under ultrasonic conditions to produce a sonicated solution,
(c) stirring the sonicated solution for a sufficient duration to produce a mixture,
(d) freeze-drying the mixture to produce rosemary nanoparticles having a particle size of about 99 nm.
2. The method according to claim 1 , wherein the step of adding the first solution to the second solution comprises spraying about 1 mL of the first solution into about 75 mL of boiling water at a flow rate of about 0.2 mL/min for about 5 minutes under ultrasonic power of 100 W and a frequency of 30 kHz.
3. The method according to claim 2 , wherein the mixture is stirred at about 200-800 rpm at room temperature for about 20 minutes prior to freeze-drying.