Method for detecting genes sensitive to low-level ionizing radiation
A method for detecting genes sensitive to low-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to low-level ionizing radiation, discovered in a carcinogenic entity and verified in a normal entity are detected by subjecting a cancerous AKR/J mouse and a normal ICR mouse to low-level radiation. Thymus is collected therefrom, and glycometabolism-related genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation can be accurately detected by preventing the interference of confounding variables.
1. A method for detecting a gene sensitive to an ionizing radiation, the method comprising the steps of:
irradiating a cancer-induced AKR/J mouse and an ICR mouse with a gamma radiation at a dose rate of a dose rate of 0.7 mGy/hr to a final dose of 1.7 Gy;
extracting thymi from the AKR/J mouse and the ICR mouse;
subjecting the thymi to a microarray analysis;
selecting a G6pc gene as a glucose metabolism-related gene from the microarray analysis; and
amplifying the G6pc gene and measuring an expression level of the gene.
2. The method of claim 1 , wherein the cancer is thymic cancer.
3. The method of claim 1 , further comprising the step of extracting the thymi at a time point when the mouse starts to die of the cancer.
4. The method of claim 1 , wherein the step of amplifying the G6pc gene uses primers having sequences set forth in SEQ ID NOS: 9 and 10.