Rationally-designed anti-mullerian inhibiting substance type II receptor antibodies
View Patent ↗Methods for generating hybrid antibodies are provided.
1. An isolated antibody which is immunologically specific for Mullerian Inhibiting Substance Type II Receptor (MISIIR), wherein the complementarity determining region 3 (CDR3) of either the light chain or the heavy chain is replaced with a sequence comprising
amino acids 533-550 of SEQ ID NO: 5.
2. The antibody of claim 1 , selected from the group consisting of a monoclonal antibody, a single chain Fv antibody, a diabody, a tribody, a tetrabody, a single domain antibody, a minibody, an scFv-Fc molecule, a Fab fragment, a Fab′ fragment and a F(ab′) 2 fragment.
3. The antibody of claim 2 , which is a single chain Fv antibody.
4. The antibody of claim 1 , wherein said antibody is immunologically specific for the extracellular domain of MISIIR.
5. The antibody of claim 1 , wherein said antibody has at least 90% identity with SEQ ID NO: 7, 8, or 9.
6. The antibody of claim 1 , wherein said antibody is conjugated to at least one radioisotope, purification tag, imaging agent, contrast agent, chemotherapeutic agent, or cytotoxic molecule.
7. The antibody of claim 1 , wherein said antibody has at least 90% identity with SEQ ID NO: 7.
8. The antibody of claim 7 , wherein said antibody comprises at least one mutation selected from the group consisting of K109R, R118N, A121L, L111M, S113Y, S120V, and V124Y.
9. A composition comprising at least one antibody of claim 1 and at least one pharmaceutically acceptable carrier.
10. The antibody of claim 1 conjugated to an agent which binds a type I receptor.
11. The antibody of claim 10 conjugated to an agent which binds ALK2, ALK3, or ALK6.
12. The antibody of claim 10 , wherein the agent which binds a type I receptor is an antibody which binds the type I receptor or is a ligand of the type I receptor.
13. The antibody of claim 11 , wherein the agent which binds a type I receptor is an antibody which binds ALK2, ALK3, or ALK6 or is a ligand of ALK2, ALK3, or ALK6.
14. The antibody of claim 1 , wherein said antibody comprises SEQ ID NO: 7, 8, or 9.