Inoculants including
Disclosed are inoculants that include Bacillus bacteria and induce production of volatile organic compounds (VOCs) by a plant that has been treated with the inoculant.
1. A composition for treating a plant or seeds of a plant, the composition formulated as a treatment composition in which are combined:
(a) an isolated Bacillus bacteria selected from the group consisting of:
(i) Bacillus amyloliquefaciens selected from the group consisting of Bacillus amyloliquefaciens strain AP-136 (NRRL B-50614), Bacillus amyloliquefaciens strain AP-188 (NRRL B-50615), Bacillus amyloliquefaciens strain AP-218 (NRRL B-50618), Bacillus amyloliquefaciens strain AP-219 (NRRL B-50619), and Bacillus amyloliquefaciens strain AP-295 (NRRL B-50620);
(ii) Bacillus mojavensis strain AP-209 (NRRL B-50616);
(iii) Bacillus solisalsi strain AP-217 (NRRL B-50617);
(iv) Bacillus simplex strain ABU 288 (NRRL B-50340); and
(v) mixtures thereof; and
(b) a suitable carrier;
the composition comprising 10 2 -10 12 cfu per ml carrier, and the composition inducing production of one or more volatile organic compounds (VOCs) by a plant that has been treated with the composition or that is grown from seed that has been treated with the composition.
2. The composition according to claim 1 , wherein the bacteria are selected from a group consisting of Bacillus amyloliquefaciens strain AP-136 (NRRL B-50614), Bacillus amyloliquefaciens strain AP-188 (NRRL B-50615), Bacillus amyloliquefaciens strain AP-218 (NRRL B-50618), Bacillus amyloliquefaciens strain AP-219 (NRRL B-50619), and Bacillus amyloliquefaciens strain AP-295 (NRRL B-50620).
3. The composition according to claim 1 , wherein the bacteria are Bacillus mojavensis strain AP-209 (NRRL B-50616).
4. The composition according to claim 1 , wherein the bacteria are Bacillus solisalsi strain AP-217 (NRRL B-50617).
5. The composition according to claim 1 , wherein the bacteria are Bacillus simplex strain ABU 288 (NRRL B-50340).
6. The composition according to claim 1 , wherein the carrier is selected from the group consisting of peat, wheat, bran, vermiculite, and pasteurized soil.
7. The composition according to claim 1 , wherein the one or more VOCs comprise one or more compounds selected from a group consisting of alpha-pinene, beta-pinene, beta-myrcene, cis-3-hexenyl acetate, limonene, beta-ocimene, linalool, (E)-4,8-dimethyl-1,3,7-nonatriene, methyl salicylate, decanal, cis-jasmone, caryophyllene, alpha-humulene, beta-farnesene, and mixtures thereof.
8. The composition according to claim 1 , wherein one or more VOCs reduce egg-laying of an insect on the plant.
9. The composition according to claim 1 , wherein one or more VOCs reduce feeding of an insect on the plant.
10. The composition according to claim 1 , wherein one or more VOCs attract a predator or a parasitoid to the plant.
11. A method of treating a plant, the method comprising administering the composition of claim 1 to the plant or to soil surrounding the plant.
12. The method according to claim 11 , wherein the plant is selected from the group consisting of alfalfa, rice, barley, rye, cotton, sunflower, peanut, corn, potato, sweet potato, bean, pea, lentil chicory, lettuce, endive, cabbage, brussel sprout, beet, parsnip, turnip, cauliflower, broccoli, turnip, radish, spinach, onion, garlic, eggplant pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, citrus, strawberry, grape, raspberry, pineapple, soybean, canola, oil seed rape, spring wheat, winter wheat, tobacco, tomato, sorghum, and sugarcane.
13. The method according to claim 11 , wherein the method reduces egg-laying of an insect on the plant.
14. The method according to claim 11 , wherein the method reduces feeding of an insect on the plant.
15. The method according to claim 11 , wherein the method attracts a predator insect or parasitoid to the plant.
16. A method of treating a plant seed, the method comprising treating the plant seed with the composition of claim 1 .