Anti-sPLA2-IIA antibodies and uses thereof

1. An isolated antibody comprising a variable region of a heavy chain and a variable region of a light chain, wherein:

the variable region of the heavy chain comprises the following CDRs: GYTFTS (SEQ ID NO: 1), WIFPGDGSTE (SEQ ID NO: 2) and WGITAFPLFDY (SEQ ID NO: 3);

the variable region of the light chain comprises the following CDRs: RASESVDYDGDSYMN (SEQ ID NO: 4), AASNLES (SEQ ID NO: 5) and LQSNEAPWT (SEQ ID NO: 6); and

wherein said antibody has a dissociation constant (K d ) for binding to human secretory phospholipase A2 IIA (sPLA2-IIA) less than 9×10 −10 M.

2. The isolated antibody according to claim 1 , wherein:

the amino acid sequence encoding the heavy chain variable region is SEQ ID NO: 13 and

the amino acid sequence encoding the light variable region is SEQ ID NO: 14.

3. The antibody of claim 1 , wherein the antibody is a chimeric antibody.

4. The antibody of claim 1 , wherein the antibody is labeled with a detectable label.

5. The antibody of claim 1 , wherein the antibody is humanized.

6. A composition comprising an antibody according to claim 1 .

7. A kit comprising at least one antibody according to claim 1 .

8. The kit according to claim 7 , comprising:

a first antibody comprising:

a variable region of the heavy chain comprising the following CDRs: GYTFTS (SEQ ID NO: 1), WIFPGDGSTE (SEQ ID NO: 2) and WGITAFPLFDY (SEQ ID NO: 3); and

a variable region of the light chain comprising the following CDRs: RASESVDYDGDSYMN (SEQ ID NO: 4), AASNLES (SEQ ID NO: 5) and LQSNEAPWT (SEQ ID NO: 6); and

a second antibody comprising:

a variable region of the heavy chain comprising the following CDRs: GFTFSS (SEQ ID NO: 7), AINSNGGSTY (SEQ ID NO: 8) and QGYGNFFDY (SEQ ID NO: 9); and

a variable region of the light chain comprising the following CDRs: RSSQSIVHSNGNTYLY (SEQ ID NO: 10), RVSNRFS (SEQ ID NO: 11) and FQGTHVPRT (SEQ ID NO: 12).

9. A method for detecting sPLA2-IIA in a biological sample, comprising:

contacting said biological sample with an antibody according to claim 1 and

detecting the anti-sPLA2-IIA antibody bound to sPLA2-IIA, thereby indicating the presence of sPLA2-IIA in said biological sample.

10. The method according to claim 9 , further defined as a method of performing an in vitro diagnostic or prognostic assay for determining the presence of sPLA2-IIA in a biological sample using the antibody.

11. The method according to claim 10 , wherein the assay is a sandwich ELISA using a coating antibody and a revealing antibody, wherein the coating antibody comprises:

a variable region of the heavy chain comprising the following CDRs: GYTFTS (SEQ ID NO: 1), WIFPGDGSTE (SEQ ID NO: 2) and WGITAFPLFDY (SEQ ID NO: 3); and

a variable region of the light chain comprising the following CDRs: RASESVDYDGDSYMN (SEQ ID NO: 4), AASNLES (SEQ ID NO: 5) and LQSNEAPWT (SEQ ID NO: 6).

12. The method according to claim 11 , wherein:

the variable region of the heavy chain of the revealing antibody comprises the following CDRs: GFTFSS (SEQ ID NO: 7), AINSNGGSTY (SEQ ID NO: 8) and QGYGNFFDY (SEQ ID NO: 9); and

the variable region of the light chain of the revealing antibody comprises the following CDRs: RSSQSIVHSNGNTYLY (SEQ ID NO: 10), RVSNRFS (SEQ ID NO: 11) and FQGTHVPRT (SEQ ID NO: 12).

13. The method according to claim 11 , wherein:

the amino acid sequence encoding the heavy chain variable region of the revealing antibody is SEQ ID NO: 15; and

the amino acid sequence encoding the light chain variable region of the revealing antibody is SEQ ID NO: 16.

14. An expression vector comprising at least one of SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20.

15. A hybridoma cell line producing an antibody against human secretory phospholipase A2 IIA (sPLA2-IIA) registered under CNCM 1-4587 or CNCM 1-4588.