Systems and devices for analysis of samples
Systems and methods for analysis of samples, and in certain embodiments, microfluidic sample analyzers configured to receive a cassette containing a sample therein to perform an analysis of the sample are described. The microfluidic sample analyzers may be used to control fluid flow, mixing, and sample analysis in a variety of microfluidic systems such as microfluidic point-of-care diagnostic platforms. Advantageously, the microfluidic sample analyzers may be, in some embodiments, inexpensive, reduced in size compared to conventional bench top systems, and simple to use. Cassettes that can operate with the sample analyzers are also described.
1. A method of analyzing a microfluidic sample, the method comprising the steps of:
providing a microfluidic sample analyzer comprising a housing with an opening therein, wherein a cassette is contained in the opening in the housing and, wherein the cassette or a component of the cassette includes at least one channel with a fluid sample therein;
identifying information about the cassette with an identification reader positioned on or within the housing;
processing information input by a user into a user interface positioned within the housing of the sample analyzer;
pressurizing the at least one channel in the cassette with a pressure-control system positioned within the housing to move the sample through the at least one channel;
accumulating an opaque material on a portion of a surface of a channel within a first measurement zone of the cassette;
activating an optical system that passes light from a first light source positioning within the housing through the first measurement zone of the cassette;
detecting the amount of light transmission through the opaque material within the first measurement zone of the cassette with a first detector of the optical system positioned within the housing opposite the first light source; and
analyzing the sample in the cassette with a control system positioned within the housing which communicates with the identification reader, the user interface, the pressure-control system, and the optical system.
2. A method as in claim 1 , comprising heating the cassette with a temperature regulating system positioned within the housing of the sample analyzer.
3. A method as in claim 1 , wherein a fluid connector fluidically interconnects first and second unconnected channels of the cassette so as to cause fluid communication between the first and second channels.
4. A method as in claim 3 , wherein, prior to first use of the cassette, at least one of the first and second channels contains a stored reagent, the cassette being sealed prior to first use so as to store the reagent in the cassette for at least one day.
5. A method as in claim 4 , wherein the stored reagent is a liquid.
6. A method as in claim 4 , wherein, prior to first use of the cassette, at least one of the first and second channels contains at least a first and a second fluid reagent which are separated by a third fluid substantially immiscible with both the first and second fluids.
7. A method as in claim 3 , wherein the fluid sample is contained within the fluid connector.
8. A method as in claim 1 , wherein the identifying information comprises identifying at least one of lot number, calibration information, and expiration date of the cassette.
9. A method as in claim 1 , wherein the first measurement zone of the cassette includes a meandering channel including a plurality of segments, and wherein the optical system is positioned adjacent more than one segments of the meandering channel.
10. A method as in claim 9 , wherein detecting comprises measuring a single signal through the more than one segments of the meandering channel.
11. A method as in claim 1 , wherein the cassette includes a plurality of measurement zones fluidically connected in series, each measurement zone aligned with an optical system and a light source positioned within the housing, the method comprising flowing the fluid sample across each of the plurality of measurement zones and measuring light transmission through each of the plurality of measurement zones.
12. A method as in claim 1 , comprising, during essentially the entire analysis, applying a substantially constant non-zero pressure drop between an inlet to the first measurement zone of the cassette and an outlet positioned downstream of the first measurement zone.
13. A method as in claim 1 , wherein the analyzer comprises a plurality of light sources including at least a first light source and a second light source adjacent the first light source, wherein the first light source is configured to pass light through a first measurement zone of the cassette and the second light source is configured to pass light through a second region of the cassette adjacent the first measurement zone, the method comprising activating the first light source when the second light source is not activated, and not activating the second light source unless the first light source is deactivated.
14. A method as in claim 13 , comprising activating the plurality of light sources sequentially with only one light source activated at a time.
15. A method as in claim 1 , wherein the opaque material comprises silver.
16. A method as in claim 1 , wherein the opaque material is formed by electroless deposition.
17. A method as in claim 1 , wherein the opaque material is deposited by electroless deposition on a metal colloid.
18. A method as in claim 17 , wherein the metal colloid comprises a gold-conjugated antibody.
19. A method as in claim 1 , wherein the opaque material is formed by flowing a metal solution through the channel.
20. A method as in claim 1 , comprising quantitatively determining the opacity of the opaque material.
21. A method as in claim 1 , comprising absorbing a fluid in the cassette with an absorbent material contained in a liquid containment region in fluid communication with the first measurement zone.
22. A method as in claim 1 , comprising absorbing substantially all liquids flowing in the cassette in a liquid containment region in fluid communication with the first measurement zone, while allowing any gases to escape from an outlet of the cassette.
23. A method as in claim 1 , wherein the fluid sample comprises whole blood.
24. A method of analyzing a microfluidic sample, the method comprising the steps of:
providing a microfluidic sample analyzer comprising a housing with an opening therein, wherein a cassette is contained in the opening in the housing and, wherein the cassette or a component of the cassette includes at least one channel with a fluid sample therein;
identifying information about the cassette with an identification reader positioned on or within the housing;
processing information input by a user into a user interface positioned within the housing of the sample analyzer;
pressurizing the at least one channel in the cassette with a pressure-control system positioned within the housing to move the sample through the at least one channel;
activating an optical system comprising a plurality of light sources including at least a first light source and a second light source adjacent the first light source, wherein the first light source is configured to pass light through a first measurement zone of the cassette and the second light source is configured to pass light through a second region of the cassette adjacent the first measurement zone, the method comprising activating the first light source when the second light source is not activated, and not activating the second light source unless the first light source is deactivated;
detecting the amount of light transmission through the first measurement zone of the cassette with a first detector of the optical system positioned within the housing opposite the first light source; and
analyzing the sample in the cassette with a control system positioned within the housing which communicates with the identification reader, the user interface, the pressure-control system, and the optical system.
25. A method of analyzing a microfluidic sample, the method comprising the steps of:
providing a microfluidic sample analyzer comprising a housing with an opening therein, wherein a cassette is contained in the opening in the housing and, wherein the cassette or a component of the cassette includes at least one channel with a fluid sample therein;
identifying information about the cassette with an identification reader positioned on or within the housing;
processing information input by a user into a user interface positioned within the housing of the sample analyzer;
pressurizing the at least one channel in the cassette with a pressure-control system positioned within the housing to move the sample through the at least one channel;
accumulating an opaque material on a portion of a surface of a channel within the first measurement zone of the cassette, wherein the opaque material comprises a metal;
activating an optical system that passes light from a first light source positioning within the housing through the first measurement zone of the cassette;
detecting the amount of light transmission through the opaque material within the first measurement zone of the cassette with a first detector of the optical system positioned within the housing opposite the first light source; and
analyzing the sample in the cassette with a control system positioned within the housing which communicates with the identification reader, the user interface, the pressure-control system, and the optical system.