Antibodies to risperidone and use thereof
Disclosed is an antibody which binds to risperidone, which can be used to detect risperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of risperidone, including multiplex detection of aripiprazole, quetiapine, olanzapine, and risperidone in a single lateral flow assay device.
1. An isolated antibody or a binding fragment thereof, which binds to risperidone, comprising:
a) a light chain variable region consisting of SEQ ID NO:3 and a heavy chain variable region consisting of SEQ ID NO:4;
b) a light chain variable region consisting of SEQ ID NO:68, SEQ ID NO:70, or SEQ ID NO:72 and a heavy chain variable region consisting of SEQ ID NO:58, SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:64, or SEQ ID NO:66;
c) a light chain variable region consisting of: SEQ ID NO:76 or SEQ ID NO:78 and a heavy chain variable region consisting of SEQ ID NO:74;
d) a light chain variable region consisting of SEQ ID NO:86, SEQ ID NO:88, or SEQ ID NO:90 and a heavy chain variable region consisting of SEQ ID NO:80, SEQ ID NO:82, or SEQ ID NO:84;
e) a light chain variable region consisting of SEQ ID NO:94 and a heavy chain variable region consisting of SEQ ID NO:92; or
f) a light chain variable region consisting of SEQ ID NO:100 and a heavy chain variable region consisting of SEQ ID NO:96 or SEQ ID NO:98.
2. The isolated antibody of claim 1 , comprising the light chain variable region consisting of SEQ ID NO:3 and the heavy chain variable region consisting of SEQ ID NO:4.
3. The isolated antibody of claim 1 , comprising the light chain variable region consisting of SEQ ID NO:68, SEQ ID NO:70, or SEQ ID NO:72 and the heavy chain variable region consisting of SEQ ID NO:58, SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:64, or SEQ ID NO:66.
4. The isolated antibody of claim 1 , comprising the light chain variable region consisting of SEQ ID NO:76 or SEQ ID NO:78 and the heavy chain variable region consisting of SEQ ID NO:74.
5. The isolated antibody of claim 1 , comprising the light chain variable region consisting of SEQ ID NO:86, SEQ ID NO:88, or SEQ ID NO:90 and the heavy chain variable region consisting of SEQ ID NO:80, SEQ ID NO:82, or SEQ ID NO:84.
6. The isolated antibody of claim 1 , comprising the light chain variable region consisting of SEQ ID NO:94 and the heavy chain variable region consisting of SEQ ID NO:92.
7. The isolated antibody of claim 1 , comprising the light chain variable region consisting of SEQ ID NO:100 and the heavy chain variable region consisting of SEQ ID NO:96 or SEQ ID NO:98.
8. The isolated antibody of claim 1 , comprising
a light chain CDR1 sequence comprising amino acid residues 44 to 60 of SEQ ID NO:3,
a light chain CDR2 sequence comprising amino acid residues 76 to 82 of SEQ ID NO:3,
a light chain CDR3 sequence comprising amino acid residues 115 to 123 of SEQ ID NO:3,
a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:4,
a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:4, and
a heavy chain CDR3 sequence comprising amino acid residues 118 to 122 of SEQ ID NO:4.
9. The isolated antibody of claim 1 , wherein the light chain variable region comprises:
a) a light chain CDR1 sequence comprising amino acid residues 44 to 54 of SEQ ID NO:68, a light chain CDR2 sequence comprising amino acid residues 70 to 76 of SEQ ID NO:68, and a light chain CDR3 sequence comprising amino acid residues 109 to 117 of SEQ ID NO:68;
b) a light chain CDR1 sequence comprising amino acid residues 44 to 58 of SEQ ID NO:70, a light chain CDR2 sequence comprising amino acid residues 74 to 80 of SEQ ID NO:70, and a light chain CDR3 sequence comprising amino acid residues 113 to 121 of SEQ ID NO:70; or
c) a light chain CDR1 sequence comprising amino acid residues 44 to 58 of SEQ ID NO:72, a light chain CDR2 sequence comprising amino acid residues 74 to 80 of SEQ ID NO:72, and a light chain CDR3 sequence comprising amino acid residues 113 to 121 of SEQ ID NO:72,
and wherein the heavy chain variable region comprises:
a) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:58, a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:58, and a heavy chain CDR3 sequence comprising amino acid residues 118 to 125 of SEQ ID NO:58;
b) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:60, a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:60, and a heavy chain CDR3 sequence comprising amino acid residues 118 to 128 of SEQ ID NO:60;
c) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:62, a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:62, and a heavy chain CDR3 sequence comprising amino acid residues 118 to 128 of SEQ ID NO:62;
d) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:64, a heavy chain CDR2 sequence comprising amino acid residues 69 to 87 of SEQ ID NO:64, and a heavy chain CDR3 sequence comprising amino acid residues 120 to 127 of SEQ ID NO:64; or
e) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:66, a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:66, and a heavy chain CDR3 sequence comprising amino acid residues 118 to 126 of SEQ ID NO:66.
10. The isolated antibody of claim 1 , wherein the light chain variable region comprises:
a) a light chain CDR1 sequence comprising amino acid residues 44 to 54 of SEQ ID NO:76, a light chain CDR2 sequence comprising amino acid residues 70 to 76 of SEQ ID NO:76, and a light chain CDR3 sequence comprising amino acid residues 109 to 117 of SEQ ID NO:76; or
b) a light chain CDR1 sequence comprising amino acid residues 46 to 55 of SEQ ID NO:78, a light chain CDR2 sequence comprising amino acid residues 71 to 77 of SEQ ID NO:78, and a light chain CDR3 sequence comprising amino acid residues 110 to 117 of SEQ ID NO:78; and wherein the heavy chain variable region comprises
a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:74,
a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:74, and
a heavy chain CDR3 sequence comprising amino acid residues 118 to 126 of SEQ ID NO:74.
11. The isolated antibody of claim 1 , wherein the light chain variable region comprises:
a) a light chain CDR1 sequence comprising amino acid residues 43 to 58 of SEQ ID NO:86, a light chain CDR2 sequence comprising amino acid residues 74 to 80 of SEQ ID NO:86, and a light chain CDR3 sequence comprising amino acid residues 113 to 121 of SEQ ID NO:86;
b) a light chain CDR1 sequence comprising amino acid residues 44 to 59 of SEQ ID NO:88, a light chain CDR2 sequence comprising amino acid residues 75 to 81 of SEQ ID NO:88, and a light chain CDR3 sequence comprising amino acid residues 114 to 122 of SEQ ID NO:88; or
c) a light chain CDR1 sequence comprising amino acid residues 44 to 54 of SEQ ID NO:90, a light chain CDR2 sequence comprising amino acid residues 70 to 76 of SEQ ID NO:90, and a light chain CDR3 sequence comprising amino acid residues 109 to 117 of SEQ ID NO:90,
and wherein the heavy chain variable region comprises:
a) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:80, a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:80, and a heavy chain CDR3 sequence comprising amino acid residues 118 to 125 of SEQ ID NO:80;
b) a heavy chain CDR1 sequence comprising amino acid residues 44 to 53 of SEQ ID NO:82, a heavy chain CDR2 sequence comprising amino acid residues 68 to 84 of SEQ ID NO:82, and a heavy CDR3 sequence comprising amino acid residues 117 to 122 of SEQ ID NO:82; or
c) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:84, a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:84, and a heavy chain CDR3 sequence comprising amino acid residues 118 to 130 of SEQ ID NO:84.
12. The isolated antibody of claim 1 , comprising
a light chain CDR1 sequence comprising amino acid residues 44 to 60 of SEQ ID NO:94,
a light chain CDR2 sequence comprising amino acid residues 76 to 82 of SEQ ID NO:94,
a light chain CDR3 sequence comprising amino acid residues 115 to 123 of SEQ ID NO:94,
a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:92,
a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:92, and
a heavy chain CDR3 sequence comprising amino acid residues 118 to 124 of SEQ ID NO:92.
13. The isolated antibody of claim 1 , comprising
a light chain CDR1 sequence comprising amino acid residues 44 to 54 of SEQ ID NO:100,
a light chain CDR2 sequence comprising amino acid residues 70 to 76 of SEQ ID NO:100, and
a light chain CDR3 sequence comprising amino acid residues 109 to 117 of SEQ ID NO:100,
and wherein the heavy chain variable region comprises
a) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:96,
a heavy chain CDR2 sequence comprising amino acid residues 69 to 87 of SEQ ID NO:96, and
a heavy chain CDR3 sequence comprising amino acid residues 120 to 128 of SEQ ID NO:96; or
b) a heavy chain CDR1 sequence comprising amino acid residues 45 to 54 of SEQ ID NO:98, a heavy chain CDR2 sequence comprising amino acid residues 69 to 85 of SEQ ID NO:98, and a heavy chain CDR3 sequence comprising amino acid residues 118 to 127 of SEQ ID NO:98.
14. The isolated antibody of claim 1 , wherein the antibody binding fragment is selected from the group of fragments consisting of Fv, F(ab′), F(ab′)2, scFv, minibody and diabody fragments.
15. The isolated antibody of claim 1 , wherein the antibody is a monoclonal antibody.
16. An assay kit comprising the antibody of claim 1 .
17. An assay device comprising a lateral flow assay device and the antibody of claim 1 , wherein the antibody is disposed on the lateral flow assay device.
18. The assay device of claim 17 , wherein the assay device is a non-porous lateral flow assay device.
19. The assay device of claim 17 , wherein the device is a porous lateral flow assay device.
20. A method of detecting risperidone in a sample, the method comprising:
(i) contacting the sample with the antibody of claim 1 labeled with a detectable marker, wherein the labeled antibody and risperidone present in the sample form a labeled complex; and
(ii) detecting the labeled complex, thereby detecting risperidone in the sample.
21. A competitive immunoassay method for detecting risperidone in a sample, the method comprising:
(i) contacting the sample with the antibody of claim 1 , and with risperidone or a competitive binding partner of risperidone, wherein one of the antibody and the risperidone or competitive binding partner thereof is labeled with a detectable marker, and wherein sample risperidone competes with the risperidone or competitive binding partner thereof for binding to the antibody to form a complex;
(ii) detecting the amount of the detectable marker bound to the complex; and
(iii) determining the amount of sample risperidone based on the amount of bound detectable marker.
22. The method of claim 21 , wherein the risperidone or competitive binding partner thereof is labeled with the detectable marker.
23. The method of claim 21 , wherein the antibody is labeled with a detectable marker.
24. The method of claim 21 , wherein the immunoassay is performed on a lateral flow assay device and the sample is applied to the device.
25. The method of claim 20 or 21 , further comprising detecting the presence of one or more analytes in addition to risperidone.
26. The method of claim 25 , wherein the one or more analytes are anti-psychotic drugs other than risperidone.
27. The method of claim 26 , wherein the anti-psychotic drugs other than risperidone are selected from the group consisting of: paliperidone, quetiapine, olanzapine, aripiprazole, and metabolites thereof.