Fluorescence module with a plurality of filters and light sources
Aspects of the subject technology provide a fluorescence module that is configured to provide a selectable light source for fluorescence microscopy, e.g., through independent paired selection of a light source (emitter) and a corresponding light filter. In some implementations, light source and/or light filter selection is controlled through the independent actuation of light sources and/or light filters on a supporting turret structure.
1. A fluorescence module configured for providing illumination to a microscope specimen, the fluorescence module comprising:
a plurality of light sources mounted on a light turret and configured to emit light along a light path; and
a plurality of light filters configured to receive the light in the light path, wherein the plurality of light filters are configured to be actuated independently from the plurality of light sources, and wherein rotatable actuation of the light turret directs the light path toward at least one of the plurality of light filters.
2. The fluorescence module of claim 1 , wherein the plurality of light filters are mounted on a filter turret, and
wherein rotatable actuation of the filter turret facilitates placement of at least one of the light filters into the light path.
3. The fluorescence module of claim 2 , further comprising:
an optical sensor configured to verify proper rotation of the filter turret for selection of at least one of the light filters.
4. fluorescence module of claim 1 , further comprising:
an optical guide comprising one or more mechanically actuatable mirrors, the optical guide configured for reflecting, using the one or more mechanically actuatable mirrors, the light emitted from at least one of the plurality of light sources and directing the light along the light path.
5. The fluorescence module of claim 1 , further comprising:
a mirror cube for receiving an emission light path originating from the specimen, and
wherein the mirror cube is configured for directing the emission light path toward one or more of: a charge coupled device (CCD) sensor, or a photodetector, a complementary metal-oxide semiconductor (CMOS) camera, a color camera, or a bayer mosaic camera.
6. The fluorescence module of claim 1 , wherein each of the light filters comprises one or more of: an excitation filter, an emission filter, or a dichoric mirror.
7. The fluorescence module of claim 1 , wherein each of the plurality of light sources comprises one or more light emitting diodes (LEDs).
8. A fluorescence module configured for providing illumination to a microscope specimen, the fluorescence module comprising:
a plurality of light sources configured to emit light along a light path, wherein each of the plurality of light sources are mounted on a light turret, the light turret configured for rotation in a first planar direction; and
a plurality of light filters configured to receive the light in the light path, wherein the plurality of light filters are configured to be actuated independently from the plurality of light sources, and wherein each of the plurality of light filters are mounted on a filter turret, the filter turret configured for rotation in a second planar direction.
9. The fluorescence module of claim 8 , wherein the first planar direction is substantially parallel to the second planar direction.
10. The fluorescence module of claim 8 , wherein the first planar direction is substantially perpendicular to the second planar direction.