Metastasis specific splice variants of Mena and uses thereof in diagnosis, prognosis and treatment of tumors
Methods and kits for diagnosis, prognosis and treatment of metastatic tumors are provided where the metastatic tumor is characterized by changes in expression of +++, ++ and/or 11a variants of Mena.
1. A kit comprising a nucleic acid encoding the +++ isoform of Mena, wherein the nucleic acid consists of SEQ ID NO: 3, or the entire complement thereof, wherein the nucleic acid is labeled with a fluorescent label or a radiolabel attached to the nucleic acid.
2. The kit of claim 1 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding Mena 11a, wherein Mena 11a consists of SEQID NO: 24.
3. The kit of claim 2 , wherein the nucleic acid encoding Mena 11a consists of SEQ ID NO:25.
4. The kit of claim 1 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding pan Mena.
5. The kit of claim 1 , further comprising a forward primer for the ++ isoform of Mena that consists of SEQ ID NO: 7 and a reverse primer for the ++ isoform of Mena that consists of SEQ ID NO: 8.
6. The kit of claim 1 , further comprising a forward primer for Mena 11a that consists of SEQ ID NO: 12 or 13, and a reverse primer for Mena 11a that consists of SEQ ID NO: 23.
7. The kit of claim 1 , further comprising a forward primer for pan Mena that consists of SEQ ID NO: 16 and a reverse primer for pan Mena consists of SEQ ID NO: 17.
8. The kit of claim 1 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding the ++ isoform of Mena, wherein the ++ isoform of Mena consists of SEQ ID NO: 2.
9. The kit of claim 8 , wherein the nucleic acid encoding the ++ isoform of Mena consists of SEQ ID NO: 1.
10. A kit comprising a pair of primers wherein the forward primer for the +++ isoform of Mena consists of SEQ ID NO: 7 and the reverse primer consists of SEQ ID NO: 10, wherein the nucleic acid is labeled with a fluorescent label or a radiolabel attached to the nucleic acid.
11. The kit of claim 10 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding Mena 11a, wherein Mena 11a consists of SEQ ID NO: 24.
12. The kit of claim 11 , wherein the nucleic acid encoding Mena 11a consists of SEQ ID NO:25.
13. The kit of claim 10 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding pan Mena.
14. The kit of claim 10 , further comprising a forward primer for the ++ isoform of Mena that consists of SEQ ID NO: 7 and a reverse primer for the ++ isoform of Mena that consists of SEQ ID NO: 8.
15. The kit of claim 10 , further comprising a forward primer for Mena 11a that consists of SEQ ID NO: 12 or 13, and a reverse primer for Mena 11a that consists of SEQ ID NO: 23.
16. The kit of claim 10 , further comprising a forward primer for pan Mena that consists of SEQ ID NO: 16 and a reverse primer for pan Mena consists of SEQ ID NO: 17.
17. The kit of claim 10 , further comprising a probe or PCR primers that specifically hybridize to nucleic acid encoding the ++ isoform of Mena, wherein the ++ isoform of Mena consists of SEQ ID NO: 2.
18. The kit of claim 17 , wherein the nucleic acid encoding the ++ isoform of Mena consists of SEQ ID NO: 1.