3-epimerase
A protein comprising a polypeptide sequence having at least 70% sequence identity to SEQ ID NO:6, SEQ ID NO:2 or SEQ ID NO:4. The protein has ketose 3-epimerase activity.
1. A protein comprising an amino acid sequence having 90% to 99% sequence identity to the amino acid sequence of SEQ ID NO: 6, wherein at least one amino acid within the amino acid sequence of the protein is substituted with a different amino acid at the corresponding position of the amino acid sequence of SEQ ID NO: 6, wherein the protein has allulose 3-epimerase activity, and wherein the amino acid sequence of the protein does not comprise the amino acid sequence of SEQ ID NO: 6.
2. The protein of claim 1 , wherein the protein is immobilized on a solid substrate.
3. The protein of claim 1 , wherein the protein comprises an amino acid sequence having 90% to 95% sequence identity to the amino acid sequence of SEQ ID NO: 6.
4. The protein of claim 3 , wherein the protein is immobilized on a solid substrate.
5. A nucleic acid molecule comprising a nucleotide sequence encoding the protein of claim 1 .
6. A vector comprising the nucleic acid molecule of claim 5 .
7. An isolated host cell comprising the nucleic acid molecule of claim 5 .
8. The isolated host cell of claim 7 , wherein the host cell is a microorganism, a mammalian cell, or a plant cell.
9. The isolated host cell of claim 8 , wherein the host cell is Escherichia coli.
10. A protein comprising the amino acid sequence of SEQ ID NO: 13, wherein the protein has allulose 3-epimerase activity.
11. A nucleic acid molecule comprising a nucleotide sequence encoding the protein of claim 3 .
12. A vector comprising t e nucleic acid molecule of claim 11 .
13. An isolated host cell comprising the nucleic acid molecule of claim 11 .
14. The isolated host cell of claim 13 , wherein the host cell is a microorganism, a mammalian cell, or a plant cell.
15. The isolated host cell of claim 14 , wherein the host cell is Escherichia coli.