Systems and methods for detecting multiple optical signals
View Patent ↗To minimize cross talk in systems and methods for detecting two or more different optical signals emitted from each of a plurality of reaction receptacles, an excitation signal associated with each of the optical signals has a known excitation frequency, and any detected signal having a frequency that is inconsistent with the excitation frequency is discarded. The receptacles are moved relative to optical sensors configured to detect each unique optical signal from an associated receptacle, and to further minimize cross talk, the optical sensors are arranged so that only one reaction receptacle at a time is in a signal detecting position with respect to one of its associated optical sensors, and the optical sensors are grouped by the optical signal they are configured to detect so that a first optical signal is detected from each of the reaction receptacles before a second optical signal is detected from the reaction receptacles.
1. A system for detecting two or more different optical emission signals emitted from each of a plurality of reaction receptacles, wherein the reaction receptacles are disposed in a fixed arrangement with respect to each other, said system comprising:
(A) a plurality of optical sensors, each of said optical sensors being configured to detect one of the two or more optical emission signals and being associated with one of the reaction receptacles so that each reaction receptacle has associated therewith one optical sensor for each optical emission signal to be detected, whereby said optical sensors associated with each of the plurality of reaction receptacles are arranged in a configuration that enables each optical sensor to detect the associated optical emission signal emitted from the associated reaction receptacle;
(B) a transport mechanism constructed and arranged to effect relative movement between the plurality of reaction receptacles and the optical sensors to sequentially place each reaction receptacle into a signal-detecting position with respect to one of its associated optical sensors, wherein
(1) each optical sensor is configured to direct an optical excitation signal at an associated reaction receptacle that is in a signal-detecting position with respect to the optical sensor and to detect an optical emission signal emitted from the reaction receptacle that is associated with the excitation signal, and
(2) each optical sensor is further configured to modulate an excitation signal at an excitation frequency and direct the modulated excitation signal at an associated receptacle, and wherein optical sensors configured to detect at least two different optical emission signals are configured to direct excitation signals of two different excitation frequencies; and
(C) circuitry configured to discard any portion of an optical signal detected by an optical sensor having a frequency that is inconsistent with the excitation frequency of the associated excitation signal.
2. The system of claim 1 , wherein said plurality of optical sensors are disposed in fixed positions, and wherein said transport mechanism comprises a receptacle transport mechanism constructed and arranged to move the reaction receptacles into signal-detecting positions with respect to said fixed optical sensors.
3. The system of claim 2 , wherein said receptacle transport mechanism comprises a rotatable carousel constructed and arranged for holding, moving, and positioning the reaction receptacles with respect to said optical sensors.
4. The system of claim 3 , wherein said optical sensors are arranged at different angular positions with respect to said carousel.
5. The system of claim 3 , wherein each of said reaction receptacles is positioned at a different radial position with respect to the axis of rotation of said carousel, and wherein the optical sensors associated with each reaction receptacle are positioned at different angular locations and at the radial position corresponding to the associated reaction receptacle.
6. The system of claim 5 , further comprising baffles configured to optically isolate each of said optical sensors from the other optical sensors, wherein said baffles comprise walls arranged concentrically and at different radial positions with respect to the axis of rotation of said carousel so as to be located between radially-adjacent reaction receptacles.
7. The system of claim 5 , wherein each of said reaction receptacles comprises a test tube, and wherein said test tubes are connected to each other in an aligned arrangement and an upright orientation and further wherein said connected arrangement of test tubes is carried on said carousel in a generally radial orientation so that each test tube is located at a different radial position.
8. The system of claim 4 , wherein said system comprises three optical sensors associated with each reaction receptacle, each of the three optical sensors being configured to detect a different one of three different optical emission signals.
9. The system of claim 8 , comprising fifteen optical sensors to detect three different optical emission signals emitted by each of at least five reaction receptacles.
10. The system of claim 9 , wherein said optical sensors are equally spaced at 24 degree increments about said carousel.
11. The system of claim 1 , comprising three optical sensors associated with each reaction receptacle, each of the three optical sensors being configured to detect a different one of three different optical emission signals.
12. The system of claim 1 , wherein each of said reaction receptacles comprises a test tube, and wherein said test tubes are connected to each other in an aligned arrangement and an upright orientation.
13. The system of claim 1 , further comprising baffles configured to optically isolate each of said optical sensors from the other optical sensors.
14. The system of claim 1 , wherein each of said optical sensors comprises a fluorometer constructed and arranged to detect fluorescent emissions from the reaction receptacles.
15. The system of claim 1 , wherein the two or more different optical signals are optical signals having different wavelengths.
16. The system of claim 1 , wherein said plurality of optical sensors are arranged so that only one reaction receptacle at a time can be in a signal-detecting position with respect to one of its associated optical sensors and optical sensors configured to detect each of the two or more optical emission signals are arranged in groups so that each of the reaction receptacles is placed in a signal-detecting position with respect to an associated optical sensor configured to detect one optical emission signal before any of the reaction receptacles is placed in signal-detecting position with respect to an associated optical sensor configured to detect a different optical emission signal.
17. The system of claim 1 , wherein the amount of each different optical signal emitted from each reaction receptacle is indicative of the presence or amount of a different target nucleic acid within the contents of the reaction receptacle, and wherein the system is configured to process detected optical emission signal data so as to determine the presence or amount, within the contents of each receptacle, of the target nucleic acid associated with each optical signal based on the detected optical emission signals.