Method of treating graft versus host disease using IL-2 muteins
Described herein are immunosuppressive molecules including immunosuppressive variants of IL-2, and use of such molecules to treat inflammatory and autoimmune disorders.
1. A method of treating graft versus host disease in a subject, said method comprising administering to a subject in need thereof a therapeutically effective amount of an IL-2 variant comprising a sequence of amino acids that differs from SEQ ID NO: 1 only at one or more of residues E15, H16, Q22, D84, N88, or E95, wherein said IL-2 variant selectively promotes FOXP3-positive regulatory T cell growth or survival in vitro.
2. The method of claim 1 , wherein the IL-2 variant induces STAT5 phosphorylation in ex vivo FOXP3-positive T cells comprising a functional IL-2 receptor complex but has a reduced ability to induce phosphorylation of STAT5 in FOXP3-negative T cells.
3. The method of claim 1 , wherein the IL-2 variant is conjugated to a chemical or polypeptide that extends the serum half-life of said IL-2 variant in vivo.