Biological sample collection and preservation
An embodiment of the claimed invention is directed to a method that greatly streamlines and reduces costs for tissue preparation, preservation, long-term storage and sample retrieval for molecular analysis using a method based on dried blood spot (DBS) technology. In this method, a small needle punch sample of freshly excised tissue will be homogenized in stabilizing reagent and inserted into a device containing absorbent material and drying agent. This device is suitable for long-term sample storage at ambient temperature and allows for easy removal of sections for biomarker analysis.
1. A method of extracting and storing a biological sample with a device, the method comprising:
retrieving into the device a tissue sample from a specimen by extending a first plunger of the device;
contacting the tissue sample with a buffer;
homogenizing the tissue sample within the device by actuating a second plunger of the device to move a homogenization device attached to the second plunger to produce a liquid homogenate within the device;
transferring the liquid homogenate on to an absorbent material; and
drying the liquid homogenate on the absorbent material to create a storable sample at ambient temperature.
2. The method of claim 1 , further comprising the step of subjecting a portion of the storable sample to testing.
3. The method of claim 1 , wherein the homogenization device is selected from the group consisting of a grating device, a friction-based device, and a blade-based device.
4. The method of claim 1 , wherein the buffer includes at least one aqueous buffer selected from the group consisting of: a phosphate buffer, a PBS containing protease inhibitor, an RNAse inhibitor, a detergent, and an organic solvent.