Method for extracorporeal removal of pathogenic microbe, an inflammatory cell or an inflammatory protein from blood
The present invention relates to a method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from mammalian blood/use of a device comprising a carbohydrate immobilized on a solid substrate, said carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, for extracorporeal removal of said pathogenic microbe, inflammatory cell or inflammatory protein from mammalian blood/use of a carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, wherein said carbohydrate is immobilized on a solid substrate, in the preparation of a device for treatment of a condition caused or aggravated by said pathogenic microbe, inflammatory cell or inflammatory protein and a method for treatment of a mammalian subject suffering from a condition caused or aggravated by a pathogenic microbe, an inflammatory cell or an inflammatory protein.
1. A method for treating a subject in need thereof, by extracorporeal removal of a bacterium or pro-inflammatory cytokine, said method comprising:
a) contacting said subject's whole blood with heparin immobilized on a solid substrate, said heparin having a terminal residue, wherein heparin immobilization consists of a single covalent link of said terminal residue to said solid substrate by covalent end-point attachment, under conditions allowing binding of said bacterium or pro-inflammatory cytokine in said subject's whole blood sample to the heparin;
b) separating the whole blood from the solid substrate;
c) recovering said whole blood containing a reduced amount of said bacterium or pro-inflammatory cytokine; and
d) reintroducing into said subject said whole blood containing a reduced amount of said a bacterium or pro-inflammatory cytokine.
2. The method of claim 1 , wherein said solid substrate comprises microparticles.
3. The method of claim 1 , wherein said solid substrate comprises fibers.
4. The method of claim 1 , wherein the material of said solid substrate is at least one member selected from the group consisting of glass, cellulose, cellulose acetate, chitin, chitosan, crosslinked dextran, crosslinked agarose, polypropylene, polyethylene, polysulfone, polyacrylonitrile, silicone, Teflon and polyurethanes.
5. The method of claim 1 , wherein said bacterium is selected from the group consisting of Streptococcus sanguis, Streptococcus mutans, Escherichia coli, Pseudomonas aureginosa and Mycobacterium tuberculosis.
6. The method of claim 1 , wherein said pro-inflammatory cytokine is selected from the group consisting of tumor necrosis factor alpha (TNF-α), tumor necrosis factor beta (TNF-β), interleukin-1 (IL-1), and interleukin-6 (IL-6).