Detecting neoplasm
This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.
1. A method for characterizing a human biological sample comprising:
(a) measuring a K-ras mutation score from a human biological sample, wherein said measuring of said K-ras mutation score is measured by digital melt curve analysis;
(b) measuring a methylation level of a CpG site of from the human biological sample;
(c) determining whether or not the human biological sample has an elevated K-ras mutation score and an elevated BMP3 methylation status as compared to a control from a subject that does not have premalignant or malignant colorectal cancer.
2. The method of claim 1 , wherein the human biological sample is a stool sample.
3. A method for characterizing a human stool sample comprising:
(a) measuring a K-ras mutation score from a human stool sample;
(b) measuring a methylation level of a CpG site of BMP3 from the human stool sample;
(c) determining whether or not the stool biological sample has an elevated K-ras mutation score and an elevated BMP3 methylation status as compared to a control from a subject that does not have premalignant or malignant colorectal cancer.
4. The method of claim 3 , wherein said measuring of said K-ras mutation score is measured by digital melt curve analysis.
5. The method of claim 3 , wherein said measuring of said K-ras mutation score is measured by quantitative allele-specific PCR.