Microfluidic process for treating and analysing a solution containing a biological material and corresponding microfluidic circuit
Provided herein are microfluidic circuits that include at least one device for forming a quantity of drops of a solution in a carrier fluid and at least one storage zone for storing drops produced by the microfluidic device. Such microfluidic circuits are useful, for example, for the analysis of a solution containing a biological sample.
1. A microfluidic circuit, wherein microchannels suitable for containing fluids are defined, said circuit comprising at least one device for forming a plurality of drops of a solution in a carrier fluid, and at least one storage zone for storing drops produced by said microfluidic circuit;
wherein said at least one device for forming drops further comprises said microchannels, wherein each of said microchannels comprise microchannel walls, said microchannel walls comprising diverging wall portions diverge, to detach drops of said solution under the effect of the surface tension of said solution to form said plurality of drops;
wherein said storage zone comprises an upper surface, a lower surface and storage zone walls, wherein each said storage zone wall comprises a rectangular shaped step;
wherein said at least one device for forming drops opens onto said step of said at least one storage zone,
wherein said diverging wall portions of said microchannel walls causes at least a portion of said plurality of drops to move to said at least one storage zone;
wherein the dimensions of said at least one storage zone are adapted to the dimensions and the quantity of the drops contained in said portion to concentrate said portion of drops;
wherein said upper surface and lower surface are spaced further from each other than said microchannel walls.
2. The microfluidic circuit according to claim 1 , wherein said microfluidic circuit further comprises at least two separate storage zones.
3. The microfluidic circuit according to claim 1 , wherein said microfluidic circuit further comprises at least two devices for forming drops comprising drop-forming nozzles with different sections; each being suitable for forming drops of different volumes.
4. The microfluidic circuit according to claim 1 , wherein said diverging wall portions for guiding the drops further comprises different inclined zones guiding said drops, wherein said drops are of different volumes, to separate storage zones.
5. The microfluidic circuit according to claim 1 , wherein at least one of said storage zones is a hole of a diameter between 10% and 120% of the diameter of a member of said drops, said hole being adapted to receive only one drop.
6. The microfluidic circuit according to claim 1 , wherein at least one of the storage zones further comprises predetermined dimensions adapted to receive said drops in a same layer.
7. The microfluidic circuit according to claim 1 , wherein at least one of the storage zones further comprises a predetermined distance between its upper surface and its lower surface, said predetermined distance of said at least one storage zone sufficient to allow the drops contained in said at least one storage zone on at least two superimposed layers.
8. The microfluidic circuit according to claim 1 , further comprising, at least in part, a transparent material suitable for viewing at least one of the storage zones, from outside the circuit.