Production of cannabinoids in yeast
Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
1. A method for making cannabigerolic acid, the method comprising:
transforming S. cervisiae with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.2 expressing an acyl-activating enzyme and expressing a mutant prenyltransferase; and
transforming the S. cervisiae with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.13 expressing olivetolic synthase, expressing olivetolic acid cyclase and expressing aromatic prenyltransferase.
2. A method for making cannabigerolic acid, the method comprising:
transforming a S. cervisiae with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.1 expressing an acyl-activating enzyme;
transforming the S. cervisiae with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.22 expressing a mutant prenyltransferase;
transforming the S. cervisiae with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.10 expressing olivetolic synthase;
transforming the S. cervisiae with a fourth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.5 expressing olivetolic acid cyclase; and
transforming the S. cervisiae with a fifth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.13 expressing aromatic prenyltransferase.
3. A method for making cannabigerolic acid, the method comprising:
transforming S. cervisiae with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.23 expressing an acyl-activating enzyme;
transforming the S. cervisiae with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.24 expressing a mutant prenyltransferase;
transforming the S. cervisiae with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.25 expressing olivetolic synthase;
transforming the S. cervisiae with a fourth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.26 expressing olivetolic acid cyclase; and
transforming the S. cervisiae with a fifth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.27 expressing aromatic prenyltransferase.