Biological specimen handling apparatus and method
In accordance with one aspect of the invention, a mold is provided for receiving a tissue specimen and molten embedding material that improves the ease of withdrawing the embedded tissue specimen. The mold has a barrier coating intimately bonded to one or more surfaces of the mold that repels the attraction of the embedding material to the one or more mold surfaces and produces a positive meniscus of the embedding material in the mold. The barrier coating permits the mold to be re-used without needing to manually re-apply a release agent to the mold before using the mold. Further, the barrier coating permits the use of more aggressive draft angles than conventional molds by reducing the frictional engagement between the embedding material and the mold cavity surfaces.
1. A tissue embedding mold comprising a bottom wall and sidewalls defining an interior volume and a paraffin resistant material coating on the entire bottom wall and sidewalls, wherein the sidewalls are oriented at a draft angle relative to the bottom wall and the draft angle is in a range of zero degrees to two degrees, wherein the tissue embedding mold comprises dimensions operable for loading into a tissue sample processing machine, wherein the paraffin resistant material comprises a self-assembled monolayer of phosphates.
2. The tissue embedding mold of claim 1 , wherein the paraffin resistant material comprises an oleophobic material.
3. The tissue embedding mold of claim 1 , wherein the sidewalls comprise primary sidewalls and the bottom wall comprises a primary bottom wall and comprises a cavity including secondary sidewalls and a secondary bottom wall defining a plane different than a plane of the primary bottom wall.
4. The tissue embedding mold of claim 3 , wherein the secondary sidewalls extend at a draft angle of 0 degrees to 2 degrees relative to a perpendicular projection from the secondary bottom wall.
5. The tissue embedding mold of claim 3 , wherein the paraffin resistant material is coated on the secondary sidewalls and the secondary bottom wall.
6. The tissue embedding mold of claim 1 , wherein the paraffin resistant material comprises a glaze.
7. The tissue embedding mold of claim 1 , wherein the mold comprises stainless steel.