Method of increasing resistance against soybean rust in transgenic soybean plants
The present invention relates to a method of increasing resistance against soybean rust in transgenic plants and/or plant cells. In these plants, the content and/or the activity of an ADR-1-protein are increased in comparison to the wild-type plants not including a recombinant ADR-1-gene.
1. A method for increasing soybean rust resistance in a soybean plant or soybean plant cell, comprising introducing and expressing in a soybean plant or soybean plant cell of at least one protein encoded by:
(i) a recombinant nucleic acid having at least 95% sequence identity to the nucleotide sequence of SEQ ID NO: 1; or
(ii) a recombinant nucleic acid encoding a protein having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2 in an amount and for a period sufficient to increase soybean rust resistance in said soybean plant or soybean plant cell as compared to a wild type soybean plant or wild type soybean plant cell.
2. The method according to claim 1 , comprising:
(a) stably transforming a soybean plant cell with an expression cassette comprising a recombinant nucleic acid encoding said at least one protein in functional linkage with a promoter; and
(b) regenerating a soybean plant from the soybean plant cell.
3. The method according to claim 2 , wherein the promoter is a constitutive promoter, a pathogen-inducible promoter, or a mesophyll-specific promoter.
4. A method for the production of a transgenic soybean plant having increased resistance against soybean rust, comprising introducing and expressing in a soybean plant or soybean plant cell a nucleic acid having at least 95% sequence identity to the nucleotide sequence of SEQ ID NO: 1 or a nucleic acid encoding a protein having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2, wherein increased content and/or activity of a protein encoded by said nucleic acid confers increased resistance against soybean rust in said transgenic soybean plant or soybean plant cell as compared to a wild type soybean plant or wild type soybean plant cell.
5. A method for the production of a product, comprising:
a) growing a transgenic soybean plant obtained by the method of claim 4 ; and
b) producing a product from or by the plant and/or parts of said transgenic soybean plant.
6. The method of claim 1 , wherein the soybean rust is Phakopsora paryhizi and/or Phakopsora meibomiae.
7. The method of claim 4 , wherein the soybean rust is Phakopsora paryhizi and/or Phakopsora meibomiae.
8. The method according to claim 1 , wherein the protein is encoded by:
(i) a recombinant nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1; or
(ii) a recombinant nucleic acid encoding a protein comprising the amino acid sequence of SEQ ID NO: 2.
9. The method according to claim 4 , wherein said nucleic acid is:
(i) a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1; or
(ii) a nucleic acid encoding a protein comprising the amino acid sequence of SEQ ID NO: 2.
10. The method of claim 4 , wherein the nucleic acid is operably linked to a promoter selected from the group consisting of a constitutive promoter, a pathogen-inducible promoter, and a mesophyll-specific promoter.
11. The method of claim 4 , further comprising selecting for a transgenic soybean plant having increased resistance against soybean rust as compared to a non-transgenic control soybean plant.
12. The method of claim 11 , further comprising obtaining seeds or progeny of said transgenic soybean plant.