Parasiticidal compositions comprising an isoxazoline active agent, methods and uses thereof

1. A method for the treatment or prevention of a parasitic infestation or infection in an animal comprising administering to the animal an effective amount of a spot-on topical veterinary composition comprising:

a) at least one isoxazoline active agent of Formula (I):

wherein:

A 1 , A 2 , A 3 , A 4 , A 5 and A 6 are each CH;

B 1 and B 3 are independently CR 2 ;

B 2 is CH;

W is O;

R 1 is CF 3 ;

each R 2 is independently halogen or C 1 -C 3 -haloalkyl;

R 4 is H;

R 5 is C 1 -C 4 alkyl substituted with R 7 ;

R 7 is C 2 -C 7 alkylaminocarbonyl, C 3 -C 9 dialkylaminocarbonyl, C 2 -C 7 haloalkylaminocarbonyl or C 3 -C 9 dihaloalkylaminocarbonyl;

and

n is 2; and

b) a pharmaceutically acceptable carrier that is suitable for the application to the skin of an animal; and wherein the carrier comprises dimethyl isosorbide;

wherein the volume of the spot-on composition is from about 0.1 ml to about 5 ml and the isoxazoline active agent of Formula (I) is present in the composition in a concentration of about 0.5 to about 25% (w/v); and wherein the method has an efficacy of at least about 90% against fleas for at least about 1 month.

2. The method of claim 1 , wherein the isoxazoline is 4-[5-[3-chloro-5-(trifluoromethyl)phenyl]-4,5-dihydro-5-(trifluoromethyl)-3-isoxazolyl]-N-[2-oxo-2-[(2,2,2-trifluoroethyl)amino]ethyl]-1-naphthalenecarboxamide.

3. The method of claim 1 , wherein:

R 5 is —CH 2 C(O)NHCH 2 CF 3 ;

B 1 and B 3 are independently C—Cl, C—F or C—CF 3 .

4. The method of claim 1 , wherein the pharmaceutically acceptable carrier further comprises a diester of a dicarboxylic acid, a glycol ester, a glycol ether, a polyglycol ether, a fatty acid ester, a polyethylene glycol, polyethylene glycol ester, an oil, an alcohol, a glycerol ester, a glycerol ether, propylene glycol, ethylene glycol, a glycol carbonate or N-methylpyrrolidone, or a mixture thereof.

5. The method of claim 4 , wherein the diester of a dicarboxylic acid is a diester of a C 6 -C 16 dicarboxylic acid.

6. The method of claim 5 , wherein the diester of a C 6 -C 16 dicarboxylic acid is diethyl sebacate or diisopropyl adipate.

7. The method of claim 4 , wherein the pharmaceutically acceptable carrier comprises a propylene glycol ester, a fatty acid ester, a polyethylene glycol ester, a polyethylene glycol, an oil, a C 6 -C 20 long-chain aliphatic alcohol, a C 1 -C 8 alcohol or a glycol ether, or a combination thereof.

8. The method of claim 1 , wherein the pharmaceutically acceptable carrier further comprises a mixed ester of sucrose and acetic acid and isobutyric acid, a low melting wax, a hard fat or a block co-polymer of ethylene oxide and propylene oxide, or a combination thereof.

9. The method of claim 4 , wherein the pharmaceutically acceptable carrier comprises glycerol formal, propylene carbonate, triacetin, diethyleneglycol monoethyl ether, polyethylene glycol 400 or benzyl alcohol, or a mixture thereof.

10. The method of claim 1 , further comprising one or more additional active agent(s).

11. The method of claim 10 , wherein the one or more additional active agent(s) is an insect growth regulator, a neonicotinoid, an avermectin, a milbemycin, a pyrethroid, a phenylpyrazole, praziquantel or a depsipeptide, or a mixture thereof.

12. The method of claim 11 , wherein the additional active agent is a neonicotinoid, and wherein the neonicotinoid is nitenpyram or imidacloprid.

13. The method of claim 11 , wherein the additional active agent is an insect growth regulator.

14. The method of claim 13 , wherein the insect growth regulator is (S)-methoprene, pyriproxyfen, hydroprene, cyromazine, fluazuron, lufenuron, or novaluron.

15. The method of claim 11 , wherein the additional active agent is an avermectin, and wherein the avermectin is eprinomectin, ivermectin or selamectin.

16. The method of 10 , wherein the additional active agent is selected from the group consisting of thiabendazole, oxibendazole, mebendazole, fenbendazole, oxfendazole, albendazole, triclabendazole, febantel, levamisole, pyrantel, morantel, praziquantel, closantel, clorsulon, an amino acetonitrile active agent, and an aryloazol-2-yl cyanoethylamino active agent.

17. The method of claim 11 , wherein the additional active agent is a pyrethroid, and wherein the pyrethroid is permethrin.

18. The method of claim 11 , wherein the additional active agent is a phenylpyrazole, and wherein the phenylpyrazole is fipronil.

19. The method of claim 11 , wherein the additional active agent is a depsipeptide, and wherein the depsipeptide is emodepside.

20. The method of claim 9 , wherein the pharmaceutically acceptable carrier comprises a mixture of dimethyl isosorbide and glycerol formal.

21. The method of claim 7 , wherein the pharmaceutically acceptable carrier comprises a mixture of dimethyl isosorbide and a fatty acid ester.

22. The method of claim 4 , wherein the pharmaceutically acceptable carrier comprises a mixture of dimethyl isosorbide and a polyglycol ether.

23. The method of claim 4 , wherein the pharmaceutically acceptable carrier comprises a glycol ether selected from the group consisting of butyl diglycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, dipropylene glycol n-butyl ether, ethylene glycol monoethyl ether, ethylene glycol monomethyl ether, dipropylene glycol monomethyl ether, and diethylene glycol monoethyl ether.

24. The method of claim 1 or 20 , wherein the isoxazoline active agent of formula (I) is present in a concentration of about 1 to about 10% (w/v).

25. The method of claim 1 or 20 , wherein the isoxazoline active agent of formula (I) is present in a concentration of about 1 to about 5% (w/v).

26. The method of claim 1 or 20 , wherein the isoxazoline active agent of formula (I) is present in a concentration of about 0.5 to about 2.0% (w/v).

27. The method of claim 11 , wherein the one or more additional active agent(s) is a milbemycin, and wherein the milbemycin is moxidectin or milbemycin oxime.

28. The method of claim 11 , wherein the one or more additional active agent(s) is a combination of an avermectin and praziquantel or a combination of a milbemycin and praziquantel, and wherein in the isoxazoline active agent of formula (I):

W is O;

R 1 is CF 3 ;

B 2 is CH;

B 1 is C—Cl;

B 3 is C—CF 3 ;

Each of A 1 , A 2 , A 3 , A 4 , A 5 and A 6 is CH;

R 4 is H; and

R 5 is —CH 2 C(O)NHCH 2 CF 3 .

29. The method of claim 28 , wherein the avermectin is eprinomectin.

30. The method of claim 28 , wherein the milbemycin is moxidectin.

31. The method of claim 29 or 30 , wherein the isoxazoline active agent of formula (I) is present in a concentration of about 1 to about 10% (w/v).

32. The method of claim 29 or 30 , wherein the isoxazoline active agent of formula (I) is present in a concentration of about 1 to about 5% (w/v).

33. The method of claim 29 or 30 , wherein the isoxazoline active agent of formula (I) is present in a concentration of about 0.5 to about 2.0% (w/v).

34. The method of claim 1 , wherein the volume of the composition is between about 0.1 to about 1 ml.

35. The method of claim 1 or 29 , wherein the animal is a cat.

36. The method of claim 1 or 30 , wherein the animal is a dog.