Phytase

1. An isolated, recombinant, or synthetic polypeptide comprising the amino acid sequence SEQ ID NO:3.

2. An isolated, recombinant, or synthetic nucleic acid comprising

(a) a nucleic acid sequence selected from a group consisting of SEQ ID NO:1 and SEQ ID NO:2, wherein the nucleic acid sequence encodes a polypeptide having a phytase activity, or

(b) a nucleic acid sequence of SEQ ID NO:1, wherein the nucleic acid sequence encodes a polypeptide having a phytase activity and the polypeptide comprises an amino acid sequence of SEQ ID NO:3.

3. A composition comprising the isolated, recombinant, or synthetic polypeptide of claim 1 .

4. The composition of claim 3 , wherein the composition is:

(a) a food or a feed,

(b) a food additive, a feed additive or a dietary supplement, or

(c) a pharmaceutical.

5. The isolated, recombinant, or synthetic nucleic acid of claim 2 , wherein:

the nucleic acid sequence of SEQ ID NO:1 encodes a polypeptide having a phytase activity, and wherein the polypeptide is produced in a recombinant Pseudomonas fluorescens expression system.

6. The isolated, recombinant, or synthetic nucleic acid of claim 2 , wherein the nucleic acid sequence of SEQ ID NO:2 encodes a polypeptide having a phytase activity, wherein the polypeptide is produced in a recombinant Pichia pastoris expression system.

7. The isolated, recombinant, or synthetic polypeptide of claim 1 , wherein the polypeptide has phytase activity and is produced via intracellular expression system.

8. The isolated, recombinant, or synthetic nucleic acid of claim 2 , wherein the phytase encoded by the nucleic acid sequence is expressed intracellularly at a level of at least 7.0 g/L, 8.0 g/L, 9.0 g/L, 10.0 g/L, 11.0 g/L, 12.0 g/L, 13.0 g/L, 14.0 g/L, 15.0 g/L, 16.0 g/L, 17.0 g/L, 18.0 g/L, 19.0 g/L, 20.0 g/L, 21.0 g/L, 22.0 g/L, 23.0 g/L, 24.0 g/L, 25.0, g/L, 26.0 g/L, 27.0 g/L, 28.0 g/L, 29.0 g/L, 30.0 g/L, 31.0 g/L, 32.0 g/L, 33.0 g/L, 34.0 g/L, 35.0 g/L, 36 g/L, 37 g/L, 38 g/L, 39 g/L, or 40 g/L.

9. The phytase of claim 7 or 8 , wherein

(a) the intracellular expression is in a gram-negative bacterium,

(b) the intracellular expression of (a), wherein the gram-negative bacterium is a Pseudomonas species, E. coli species, Ralstonia species, or Caulobacter species, or

(c) the intracellular expression of (a), wherein the gram-negative bacterium is a Pseudomonas fluorescens species.

10. The phytase of claim 9 , wherein the phytase is derived from an E. coli , a Bacillus sp., a Hafnia sp., Perniophora lycii , a Buttiauxella sp., a Citrobacter sp., or Aspergillus niger.

11. The isolated, recombinant, or synthetic polypeptide of claim 1 , wherein the polypeptide

(a) does not comprise an endogenous, modified, or heterologous signal sequence, a proprotein sequence, or any combination thereof, or

(b) further comprises a heterologous amino acid sequence selected from the group consisting of: a signal sequence, a tag, an epitope, an N-terminal extension, a C-terminal extension, and any combination thereof.

12. The isolated, recombinant, or synthetic polypeptide of claim 1 , wherein the polypeptide

(a) further comprises at least a second enzyme, or

(b) the polypeptide of (a), wherein the second enzyme is selected from the group consisting of: a lactase, a lipase, a protease, a catalase, a xylanase, a cellulase, a glucanase, a mannanase, an amylase, an amidase, an epoxide hydrolase, an esterase, phospholipase, transaminase, an amine oxidase, cellobiohydrolase, an ammonia lyase, and any combination thereof.

13. The isolated, recombinant, or synthetic polypeptide of claim 1 , wherein the phytase:

(a) has a specific activity from about 1000 U/mg to 1,600 U/mg,

(b) is active at a pH from at least pH 1.0 to about pH 9.0, or

(c) is active at a temperature from about 50 degrees C. to about 100 degrees C.