Fluorogenic or fluorophoric compounds and uses thereof
Compounds are provided that are either fluorogenic or fluorophoric. Compositions and articles that include the compounds are also provided. Additionally, methods of detecting a microorganism using the compounds are provided. The compounds are fluorinated and can be used advantageously under acidic conditions.
1. A method of detecting a microorganism, the method comprising:
providing a fluorogenic compound of Formula (Ia)
wherein
R 1 is —(CO)—OR 3 , —(CO)—NR 4 R 5 , heterocyclic group, aryl, aralkyl, or cyano;
R 2a is an enzyme-labile group;
R 3 is alkyl, alkenyl, aryl, or aralkyl;
R 4 and R 5 are each independently hydrogen, alkyl, aryl, or aralkyl; and
either (a) X1 is hydrogen and X2 is fluoro or (b) X1 is fluoro and X2 is hydrogen; and
incubating a test sample with the fluorogenic compound of Formula (Ia), wherein the test sample contains an enzyme that cleaves reacts with group R 2a resulting in the formation of a fluorophoric compound of Formula (Ib); and
exciting the fluorophoric compound of Formula (Ib) with a first wavelength of light and detecting light emitted at a second wavelength of light that is longer than first wavelength of light.
2. The method of claim 1 , wherein the first wavelength of light and the second wavelength of light are in the ultraviolet, visible, or infrared region.
3. The method of claim 1 , wherein the step of incubating the test sample with the fluorogenic compound comprises adding the test sample in a liquid form to a dehydrated layer containing the fluorogenic compound of Formula (Ia) and a growth medium.
4. The method of claim 1 , wherein the method further comprises sequestering the compound of Formula (Ib) on polymeric particles.
5. The method of claim 1 , wherein the polymeric particles have an average particle diameter in a range of 0.01 micrometers to 1 micrometer.