Device for separation and collection of plasma
View Patent ↗A substrate for positioning a separation membrane and a collection membrane for separating and collecting plasma is disclosed. The substrate includes an inner flexure disposed proximate to a first peripheral portion of the substrate and an outer flexure disposed surrounding at least a portion of the inner flexure. The inner flexure is formed from a plurality of first slots in the substrate and the outer flexure is formed from a plurality of second slots in the substrate.
1. A substrate for positioning a separation membrane and a collection membrane for separating and collecting plasma, the substrate comprising:
an inner flexure defined by a plurality of first slots in the substrate, disposed proximate to a first peripheral portion of the substrate; and
an outer flexure defined by a plurality of second slots in the substrate, disposed surrounding at least a portion of the plurality of first slots,
wherein the inner flexure comprises a first distal end portion and the outer flexure comprises a second distal end portion adjacently overlapping with a fraction of the first distal end portion of the inner flexure, and means for holding and aligning the separation membrane to position along the inner flexure, said means located between the first peripheral portion and a first proximal end portion of the inner flexure.
2. The substrate of claim 1 , wherein the first distal end portion comprises a first tapered portion and the second distal end portion comprises a second tapered portion.
3. The substrate of claim 2 , wherein the first tapered portion and second tapered portion have a complementary profile.
4. The substrate of claim 1 , wherein the plurality of first slots comprises a plurality of first sub-slots and a first mid-slot, wherein the plurality of first sub-slots is proximate to the first peripheral portion and the first mid-slot is located at the first distal end portion and connected to a distal end of each first sub-slot.
5. The substrate of claim 4 , wherein the first mid-slot extends along a transverse direction and each first sub-slot extends at a predetermined angle relative to the first mid-slot.
6. The substrate of claim 4 , wherein the plurality of second slots comprises a plurality of second sub-slots and a second mid-slot, wherein the plurality of second sub-slots surrounds at least a portion of the plurality of first sub-slots, and the second mid-slot is proximate to the first mid-slot and connected to a distal end of each second sub-slot.
7. The substrate of claim 6 , wherein the second mid-slot extends along a transverse direction and each second sub-slot extends at a predetermined angle relative to the second mid-slot.
8. The substrate of claim 7 , wherein the predetermined angle is greater than or equal to 90 degrees.
9. The substrate of claim 1 , wherein the inner flexure has a first stiffness (S1) and the outer flexure has a second stiffness (S2).
10. The substrate of claim 9 , wherein the first stiffness (S1) is dependent on a geometry and a thickness of the inner flexure, and the second stiffness (S2) is dependent on a geometry and a thickness of the outer flexure such that S1 is less than S2 as measured by a degree of deflection for an applied load.
11. The substrate of claim 1 , further comprising a plurality of guiding mechanism located surrounding a portion of the plurality of second slots and proximate to a second peripheral portion of the substrate, configured to support and guide the collection membrane to position beneath the outer flexure,
wherein the plurality of guiding mechanism is a ridge having a protrusion.
12. A device for separating and collecting plasma, the device comprising:
a separation membrane;
a collection membrane; and
a substrate, wherein the substrate comprises:
an inner flexure defined by a plurality of first slots in the substrate, disposed proximate to a first peripheral portion of the substrate; and
an outer flexure defined by a plurality of second slots in the substrate, disposed surrounding at least a portion of the plurality of first slots;
wherein a distal end of the separation membrane is disposed under the outer flexure, wherein a proximal end of the collection membrane is disposed under at least one of the outer flexure and inner flexure such that the proximal end of the collection membrane has an adjacently overlapping contact area with the distal end of the separation membrane, and wherein the outer flexure is configured to apply pressure on the separation membrane and collection membrane about the adjacently overlapping contact area.
13. The device of claim 12 , wherein the adjacently overlapping contact distance in the longitudinal direction is in a range from about 1 mm to about 2 mm.
14. The device of claim 12 , further comprising a plurality of holding mechanism located between the first peripheral portion and a first proximal end portion of the inner flexure, configured to hold and align the separation membrane to position along the inner flexure.
15. The device of claim 12 , further comprising a plurality of guiding mechanism disposed surrounding a portion of the plurality of second slots and proximate to a second peripheral portion of the substrate, configured to support and guide the collection membrane to position beneath the outer flexure,
wherein the guiding mechanism is a ridge having a protrusion.
16. The device of claim 12 , wherein the separation membrane and collection membrane comprise at least one of a cellulose, a glass fiber, a cellulose acetate, a polyvinyl pyrrolidone, a polysulfone, a polyethersulfone, and a polyester.