Small-molecule inhibitors targeting G-protein-coupled Rho guanine nucleotide exchange factors
View Patent ↗Provided are inhibitors of Rho GTPase activation, and, in particular, compounds that inhibit RhoA activation by an RhoGEF. Also provided are related pharmaceutical compositions and methods. Also provided are methods of inhibiting Rho GTPase activation. Also provided are methods of screening for compounds that inhibit Rho GTPase activation by a RhoGEF.
1. A method of inhibiting Rho GTPase activation comprising contacting a RhoGEF with a compound having the structure of Formula Ia:
or a pharmaceutically acceptable salt thereof,
wherein:
R 1 is
or 1H-isoindolyl;
each R 6 is independently selected from the group consisting of hydroxy, fluoro, chloro, C 1-3 alkyl, C 1-3 alkoxy, and C 1-4 alkoxy substituted with R 1B ;
each R 1B is independently selected from the group consisting of —OR 1C , aryl, and heteroaryl, said aryl or heteroaryl each optionally substituted with one or more R 1D ;
each R 1C is independently selected from the group consisting of aryl, and heteroaryl, said aryl or heteroaryl each optionally substituted with one or more R 1E ;
each R 1D is independently selected from the group consisting of hydroxy, halo, cyano, nitro, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, amino, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl optionally substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro;
each R 1E is independently selected from the group consisting of hydroxy, halo, cyano, nitro, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, amino, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl optionally substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro;
R 2 is phenyl; and
R 3 is H (hydrogen).
2. The method of claim 1 , wherein a therapeutically effective amount of the compound having the structure of Formula I is administered to a subject in need of treatment for breast cancer, leukemia or lung cancer.
3. The method of claim 2 , where the a compound having the structure of Formula I has the structure:
or a pharmaceutically acceptable salt thereof.
4. A method of inhibiting Rho GTPase activation comprising contacting a RhoGEF with a compound having the structure of Formula I:
or a pharmaceutically acceptable salt thereof,
wherein:
R 1 is
or 1H-isoindolyl;
each R 6 is independently selected from the group consisting of fluoro, chloro, methyl, ethoxy, and C 1-3 alkoxy substituted with R 1B ;
each R 1B is independently selected from the group consisting of —OR 1C , aryl, and heteroaryl, said aryl or heteroaryl each optionally substituted with one or more R 1D ;
each R 1C is independently selected from the group consisting of aryl, and heteroaryl, said aryl or heteroaryl each optionally substituted with one or more R 1E ;
each R 1D is independently selected from the group consisting of hydroxy, cyano, nitro, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, amino, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, and C 1-6 alkoxy optionally substituted with up to 5 fluoro;
each R 1E is independently selected from the group consisting of hydroxy, halo, cyano, nitro, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, amino, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl optionally substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro;
R 2 is H (hydrogen), aryl, or heteroaryl, said aryl or heteroaryl each optionally substituted with one or more R 2A ;
each R 2A is independently selected from the group consisting of hydroxy, halo, cyano, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro; and
R 3 is H (hydrogen), aryl, or heteroaryl, said aryl or heteroaryl each optionally substituted with one or more R 3A , provided that one of R 2 and R 3 is H (hydrogen) and one of R 2 and R 3 is not H (hydrogen);
each R 3A is independently selected from the group consisting of hydroxy, halo, cyano, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro;
R 4 is H (hydrogen), or C 1-6 alkyl; and
R 5 is H (hydrogen), or C 1-6 alkyl, or optionally R 4 and R 5 together are oxo.
5. The method of claim 4 , wherein R 3 is H (hydrogen).
6. The method of claim 5 , wherein R 2 is phenyl optionally substituted with one or more R 2A ; and each R 2A is independently selected from the group consisting of halo, C 1-6 alkyl substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro.
7. The method of claim 6 , wherein R 2 is
8. The method of claim 7 , wherein R 2A is fluoro, chloro, bromo, methoxy, ethoxy, trifluoromethyl, or trifluoromethoxy.
9. The method of claim 4 , wherein R 3 is phenyl optionally substituted with one or more R 3A ; and each R 3A is independently selected from the group consisting of halo, C 1-6 alkyl substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro.
10. The method of claim 9 , wherein R 3 is
11. The method of claim 10 , wherein R 3A is fluoro, chloro, bromo, methoxy, ethoxy, trifluoromethyl, or trifluoromethoxy.
12. The method of claim 4 , where the a compound having the structure of Formula I has the structure:
or a pharmaceutically acceptable salt thereof.
13. A method of inhibiting Rho GTPase activation comprising contacting a RhoGEF with a compound having the structure of Formula Ia:
or a pharmaceutically acceptable salt thereof,
wherein:
R 1 is
or 1H-isoindolyl;
each R 6 is independently selected from the group consisting of fluoro, chloro, bromo, methyl, methoxy, and C 1-4 alkoxy substituted with R 1B ;
each R 1B is independently selected from the group consisting of —OR 1C , aryl, and heteroaryl, said aryl or heteroaryl each optionally substituted with one or more R 1D ;
each R 1C is independently selected from the group consisting of aryl, and heteroaryl, said aryl or heteroaryl each optionally substituted with one or more R 1E ;
each R 1D is independently selected from the group consisting of hydroxy, halo, cyano, nitro, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, amino, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl optionally substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro;
each R 1E is independently selected from the group consisting of hydroxy, halo, cyano, nitro, O-carbamyl, N-carbamyl, O-thiocarbamyl, N-thiocarbamyl, C-amido, N-amido, S-sulfonamido, N-sulfonamido, C-carboxy, O-carboxy, isocyanato, thiocyanato, isothiocyanato, amino, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl optionally substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro;
R 2 is aryl optionally substituted with one or more R 2A ;
each R 2A is independently selected from the group consisting of hydroxy, bromo, cyano, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro; and
R 3 is H (hydrogen);
each R 3A is independently selected from the group consisting of hydroxy, bromo, cyano, optionally substituted C 3-7 cycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted heterocyclyl, C 1-6 alkyl substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro;
R 4 is H (hydrogen), or C 1-6 alkyl; and
R 5 is H (hydrogen), or C 1-6 alkyl, or optionally R 4 and R 5 together are oxo.
14. The method of claim 13 , wherein R 3 is H (hydrogen).
15. The method of claim 14 , wherein R 2 is phenyl optionally substituted with one or more R 2A ; and each R 2A is independently selected from the group consisting of halo, C 1-6 alkyl substituted with up to 5 fluoro, and C 1-6 alkoxy optionally substituted with up to 5 fluoro.
16. The method of claim 15 , wherein R 2 is
17. The method of claim 16 , wherein R 2A is fluoro, chloro, bromo, methoxy, ethoxy, trifluoromethyl, or trifluoromethoxy.
18. The method of claim 13 , where the a compound having the structure of Formula I has the structure:
or a pharmaceutically acceptable salt thereof.
19. A method of inhibiting Rho GTPase activation comprising contacting a RhoGEF with a compound having the structure
or a pharmaceutically acceptable salt thereof.