Combination of vaccination and inhibition of the PD-1 pathway
The present invention relates to a vaccine/inhibitor combination comprising an RNA vaccine comprising at least one RNA comprising at least one open reading frame (ORF) coding for at least one antigen and a composition comprising at least one PD-1 pathway inhibitor, preferably directed against PD-1 receptor or its ligands PD-L1 and PD-L2. The present invention furthermore relates to a pharmaceutical composition and a kit of parts comprising the components of such a vaccine/inhibitor combination. Additionally the present invention relates to medical use of such a vaccine/inhibitor combination, the pharmaceutical composition and the kit of parts comprising such a vaccine/inhibitor combination, particularly for the prevention or treatment of tumor or cancer diseases or infectious diseases. Furthermore, the present invention relates to the use of an RNA vaccine in therapy in combination with a PD-1 pathway inhibitor and to the use of a PD-1 pathway inhibitor in therapy in combination with an RNA vaccine.
1. A method of treating a subject having a cancer or a tumor disease, the method comprising administering to the subject an effective amount of:
(i) an immunogenic composition comprising at least one RNA, wherein the at least one RNA is an mRNA comprising at least one open reading frame (ORF) encoding at least one tumor antigen, wherein the RNA is codon optimized for the human codon usage; and
(ii) a PD-1 pathway inhibitor, wherein the PD-1 pathway inhibitor is an antagonistic antibody, which is directed against PD-1.
2. The method of claim 1 , wherein the PD-1 pathway inhibitor is administered after said immunogenic composition.
3. The method of claim 1 , wherein the antibody directed against PD-1 is selected from the group consisting of Nivolumab (BMS-936558/MDX1106), Pidilizumab (CT-011), and MK-3475(SCH 900475).
4. The method of claim 1 , wherein the at least one RNA comprises an increased G/C content relative to a wild type RNA encoding the antigen.
5. The method of claim 1 , wherein the at least one RNA is complexed with a carrier.
6. The method of claim 5 , wherein the carrier is a cationic, polycationic or polymeric carrier.
7. The method of claim 6 , wherein the carrier comprises protamine.
8. The method of claim 1 , wherein the immunogenic composition and the PD-1 pathway inhibitor are administered sequentially.
9. The method of claim 1 , wherein the immunogenic composition and the PD-1 pathway inhibitor are administered concurrently.
10. The method of claim 1 , wherein the immunogenic composition and the PD-1 pathway inhibitor are administered via different administration routes.