Recombinant adeno-associated virus vector for treatment of Alzheimer disease
View Patent ↗Disclosed is an adeno-associated virus vector capable of expressing a peptide fragment containing a humoral immunity induction site of the β-amyloid peptide, comprising DNA encoding the peptide fragment in an operative form.
1. A method for treating Alzheimer's disease in a mammal in need thereof, comprising orally administering to the mammal a DNA vaccine composition, wherein said composition comprises an adeno-associated virus vector encoding a β-amyloid peptide and a signal peptide capable of extracellularly secreting said β-amyloid peptide, in an operative form, wherein said administering results in suppressing formation of an amyloid plaque in the central nervous system and reducing the concentration of TGF-β1 in the blood of the mammal, and wherein administering said composition maintains therapeutic activity for at least 6 months.
2. The method according to claim 1 , wherein the β-amyloid peptide is expressed in intestinal cells by the adeno-associated virus vector.
3. The method according to claim 1 , wherein said β-amyloid peptide comprises the amino acids 4 to 10 of the amino acid sequence as shown in SEQ ID NO: 2 .
4. The method according to claim 1 , wherein the DNA encoding said β-amyloid peptide comprises the nucleotides 10 to 30 of the nucleotide sequence as shown in SEQ ID NO: 1.
5. The method according to claim 1 , wherein said β-amyloid peptide comprises the amino acid sequence as shown in SEQ ID NO: 2.
6. The method according to claim 1 , wherein the DNA encoding said β-amyloid peptide comprises the nucleotide sequence as shown in SEQ ID NO: 1.
7. The method according to claim 1 , wherein said β-amyloid peptide comprises the amino acid sequence as shown in SEQ ID NO: 4.
8. The method according to claim 1 , wherein the DNA encoding said β-amyloid peptide comprises the nucleotide sequence as shown in SEQ ID NO: 3.
9. The method according to claim 1 , wherein said signal peptide is a signal peptide of amyloid precursor protein.
10. The method according to claim 1 , wherein said signal peptide comprises the amino acid sequence as shown in SEQ ID NO: 6.
11. The method according to claim 1 , wherein the DNA encoding said signal peptide comprises the nucleotide sequence as shown in SEQ ID NO: 5.
12. The method according to claim 1 , wherein progress of cerebrovascular amyloid deposition or microvascular degeneration is suppressed.
13. The method according to claim 1 , wherein anti-β amyloid peptide antibody production is induced without substantially inducing a cellular immune response.
14. The method according to claim 13 , wherein the induced cellular immune response causes encephalitis.