Scanning microscope and method for scanning microscopy
A scanning microscope has a detector for detecting the detected light proceeding from a sample. A bandpass filter, containing a combination of a short-pass filter and at least one long-pass filter, is arranged in the light path of the detected light. The detector is a non-descan detector.
1 . A scanning microscope comprising: a non-descan detector for detecting the detected light proceeding from a sample, a bandpass filter, which comprises a combination of at least one short-pass filter and at least one long-pass filter and which is arranged in the light path of the detected light.
2 . The scanning microscope as defined in claim 1 , wherein the short-pass filter and/or the long-pass filter comprises a spectral gradient filter and/or of a dielectric filter and/or of a gradient interference filter.
3 . The scanning microscope as defined in claim 1 , wherein the short-pass filter and/or the long-pass filter comprises a spectral gradient filter and/or a gradient interference filter and at least one moveable beam stop whereby the spectral stopband of the short-pass filter and/or the one long-pass filter is defined by the relative position of the at least one beam stop to the gradient interference filter and or the spectral gradient filter.
4 . The scanning microscope as defined in claim 1 , wherein the short-pass filter and/or the long-pass filter are rotatable and/or displaceable relative to one another.
5 . The scanning microscope as defined in claim 1 , wherein generation of the detected light in the sample encompasses a second-harmonic generation (SHG) process and/or a multi-photon process.
6 . The scanning microscope as defined in claim 5 , further comprising a pulsed light source, in particular a pulsed laser, for generating the illuminating light.
7 . The scanning microscope as defined in claim 1 , wherein the light path of the detected light encompasses several detection channels.
8 . The scanning microscope as defined in claim 1 , wherein the scanning microscope is a confocal scanning microscope.
9 . A method for scanning microscopy on a sample, comprising the steps of:
ascertaining an illuminating light frequency at which detected light having the frequency of a harmonic of the illuminating light frequency can be generated in the sample;
illuminating the sample with illuminating light of the ascertained illuminating light frequency;
blocking out from the detected light proceeding from the sample those light components that have the frequency of the illuminating light and/or the frequency of autofluorescence light, using a bandpass filter that comprises a combination of a short-pass and at least one long-pass filter and is arranged in the light path of the detected light; and
detecting the filtered detected light using a non-descan detector.
10 . The method as defined in claim 9 , wherein the short-pass filter and/or the long-pass filter comprises a spectral gradient filter and/or of a dielectric filter and/or of a gradient interference filter.
11 . The method as defined in claim 9 , wherein the short-pass filter and/or the long-pass filter comprises a spectral gradient filter and/or a gradient interference filter and at least one moveable beam stop whereby the spectral stopband of the short-pass filter and/or the one long-pass filter is defined by the relative position of the at least one beam stop to the gradient interference filter and or the spectral gradient filter.
12 . The method as defined in claim 9 , wherein the short-pass filter and/or the long-pass filter are rotatable and/or displaceable relative to one another.
13 . The method as defined in claim 9 , wherein generation of the detected light in the sample encompasses a second-harmonic generation (SHG) process and/or a multi-photon process.
14 . The method as defined in claim 13 , further comprising a pulsed light source, in particular a pulsed laser, for generating the illuminating light.
15 . The method as defined in claim 9 , wherein the light path of the detected light encompasses several detection channels.
16 . The method as defined in claim 15 , wherein bandpass filters are provided in several detection channels.
17 . The method as defined in claim 15 , wherein the bandpass filter causes splitting of the detection channels.
18 . The method as defined in claim 9 , wherein a confocal scanning microscope is used to carry it out.