Method and kit for diagnosing myocardial infarction
View Patent ↗The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their ability to evidence at least one particular disease state; thereby enabling a diagnostician to gain the ability to characterize either the presence or absence of at least one disease state relative to recognition of the presence and/or the absence of the biopolymer.
1. A method for diagnosing myocardial infarction comprising:
(a) conducting a mass spectrometric analysis on a sample obtained from a patient in a manner effective to maximize elucidation of discernible peptide fragments contained therein; and
(b) comparing mass spectrum profiles of a peptide consisting of SEQ ID NO:1 to mass spectrum profiles of peptides elucidated from said sample; wherein recognition of a mass spectrum profile in the sample displaying the characteristic profile of the mass spectrum profile for the peptide consisting of SEQ ID NO:1 is diagnostic for myocardial infarction.
2. The method of 1 , wherein said sample is an unfractionated body fluid or a tissue sample.
3. The method of 1 , wherein said sample is selected from the group consisting of blood, blood products, urine, saliva, cerebrospinal fluid, and lymph.
4. The method of 1 , wherein said mass spectrometric analysis is Surface Enhanced Laser Desorption Ionization (SELDI) mass spectrometry (MS).
5. The method of 1 , wherein said patient is a human.