EPITOPE SYNCHRONIZATION IN ANTIGEN PRESENTING CELLS
Disclosed herein are vaccines and methods for inducing an immune response against cancer cells and cells infected with intracellular parasites. Vaccines having housekeeping epitopes are disclosed. The housekeeping epitope is formed by housekeeping proteasomes in peripheral cells, but not by professional antigen presenting cells. A vaccine containing a housekeeping epitope that is derived from an antigen associated with a peripheral target cell can thus direct an immune response against the target cell. Methods of treatment are also disclosed, which involve administering a vaccine having a housekeeping epitope.
1 . A method of making an immunogenic composition, comprising the steps of:
selecting a first housekeeping epitope by identifying epitopes that are or could be produced from a particular antigen source by housekeeping proteasomes, wherein the housekeeping epitope is derived from a first antigen associated with a first target cell or target pathogen; and
preparing an immunogenic composition comprising or encoding the selected housekeeping epitope.
2 . An immunogenic composition made according to the method of claim 1 .
3 . A method of treating an animal, comprising administering to the animal the composition of claim 2.