Self-addressable self-assembling microelectronic integrated systems, component devices, mechanisms, methods, and procedures for molecular biological analysis and diagnostics
View Patent ↗A method for electronically stabilizing hybridization of nucleic acids bound at a test site of a microelectronic device is described. First and second negatively charged nucleic acids are provided, the second nucleic acid being bound to the test site. A zwitterionic buffer having a conductance of less than 100 mS/cm is applied to the microelectronic device. A current is applied to the test site to positively bias the test site, such that the first negatively charged nucleic acid is transported to the positively biased test site having the bound the second negatively charged nucleic acid. At the test site, the first and second negatively charged nucleic acids hybridize. The zwitterionic buffer acquires a net positive charge under influence of the current, such that the positively charged zwitterionic buffer stabilizes the hybridization by reducing the repulsion between the first and second negatively charged nucleic acids.
1. A method for electronically stabilizing hybridization of nucleic acids bound at a test site of a microelectronic device, comprising the steps of:
providing a first negatively charged nucleic acid bound to the test site, wherein said test site is generated using a technique selected from the group consisting of a microlithographic technique and a micromachining technique;
providing a solution including a second negatively charged nucleic acid;
applying a zwitterionic buffer having a conductance of less than 100 mS/cm to the microelectronic device; and
applying current to the test site to positively bias the test site,
wherein the second negatively charged nucleic acid is transported to the positively biased test site having the first negatively charged nucleic acid,
wherein the first and second negatively charged nucleic acids hybridize,
wherein the zwitterionic buffer acquires a net positive charge under influence of the current, wherein the positively charged zwitterionic buffer stabilizes the hybridization by reducing the repulsion between the first and second negatively charged nucleic acids.
2. The method of claim 1 , wherein the zwitterionic buffer comprises histidine.
3. The method of claim 1 , wherein the isoelectric point of said zwitterionic buffer is about pH 7.47.
4. The method of claim 1 , wherein the zwitterionic buffer is cationic.
5. The method of claim 1 , wherein the zwitterionic buffer is dicationic.
6. The method of claim 1 , wherein the zwitterionic buffer is polycationic.