Compositions and methods for preserving permeation layers for use on active electronic matrix devices
View Patent ↗An improved synthetic, polymer hydrogel permeation layer for use with an active electronic matrix device for biological assays. The permeation layer includes a dried coating of raffinose or a combination of raffinose and stachyose to protect the permeation layer from degradation during shipping and storage.
1. A sugar-based coating for a permeation layer overlying an electrode on a substrate, the coating comprising a solution of raffinose and stachyose in combination.
2. The sugar-based coating of claim 1 , wherein the solution of raffinose and stachyose further comprises raffinose and stachyose in combination in an amount of between about 0.5% by weight to about 10% by weight, and wherein the permeation layer comprises a material selected from the group consisting of hydrogels and sol-gels.
3. A method of coating a permeation layer overlying an electrode on a substrate, the method comprising the steps of:
a. preparing a permeation layer
b. preparing a sugar-based preservative solution comprising raffinose and stachyose in combination in an amount of between about 0.5% by weight to about 10% by weight;
c. covering the permeation layer with an amount of preservative solution sufficient to substantially cover an outside surface of the permeation layer; and
d. allowing preservative solution to dry on the permeation layer,
wherein the permeation layer comprises a material selected from the group consisting of hydrogels and sol-gels.
4. The method of claim 3 , wherein the sugar-based preservative solution comprises about 5% raffinose by weight and about 5% stachyose by weight.
5. The method of claim 3 , wherein the sugar-based preservative solution comprises about 9% raffinose by weight and about 1% stachyose by weight.
6. The method of claim 3 , wherein the sugar-based preservative solution comprises about 0% raffinose by weight and about 10% stachyose by weight.
7. A sugar-based coating for a permeation layer overlying an electrode on a substrate, the coating comprising a solution of stachyose.
8. The sugar-based coating of claim 7 , wherein the solution of stachyose further comprises stachyose in an amount of between about 0.5% by weight to about 10% by weight.