Adhering Surfaces
The present invention relates, in part, to compositions useful for cell culture having one surface in adherence to another surface, e.g., a cell culture matrix in adherence to a surface. The present invention also relates, in part, to methods of adhering one surface to another surface, e.g., adhering a cell culture matrix to a surface, and compositions relating to such methods. The present invention also provides in part, methods for adhering a cell to a surface. Related methods are also provided for determining the effect of at least one compound on a cell(s).
1 . A composition comprising a first surface coated with a charged molecule and a second surface adhering to the first surface, wherein a cell culture matrix comprises the second surface.
2 . The composition of claim 1 , wherein the first and the second surfaces are negatively charged and the charged molecule is a positively charged molecule.
3 . The composition of claim 2 , wherein a tissue culture vessel comprises the first surface.
4 . The composition of claim 3 , wherein the positively charged molecule is a polyamine.
5 . The composition of claim 4 , wherein the polyamine is a polyallylamine.
6 . The composition of claim 5 , wherein the cell culture matrix is a sponge.
7 . The composition of claim 6 , wherein the sponge is comprised of an alginate.
8 . A method of producing a composition, the method comprising:
(a) coating a first surface with a positively charged molecule; and
(b) contacting the first surface with a second surface, wherein a cell culture matrix comprises the second surface.
9 . The method of claim 8 , wherein a tissue culture vessel comprises the first surface.
10 . The method of claim 9 , wherein the coating of the first surface comprises contacting the first surface with a positively charged molecule in a solvent.
11 . The method of claim 10 , wherein the positively charged molecule is a polyamine.
12 . The method of claim 11 , wherein the polyamine is a polyallylamine.
13 . The method of claim 12 , wherein the cell culture matrix is a sponge.
14 . The method of claim 13 , wherein the sponge is comprised of an alginate.
15 . A method of culturing cells on a cell culture matrix, the method comprising:
(a) coating a first surface with a positively charged molecule;
(b) contacting the first surface with a second surface, wherein a cell culture matrix comprises the second surface; and
(c) contacting the cells with the cell culture matrix under conditions suitable for culturing the cells.
16 . The method of claim 15 , wherein a tissue culture vessel comprises the first surface.
17 . The method of claim 16 , wherein the coating of the first surface comprises contacting the first surface with a positively charged molecule in a solvent.
18 . The method of claim 17 , wherein the positively charged molecule is a polyamine.
19 . The method of claim 18 , wherein the polyamine is a polyallylamine.
20 . The method of claim 19 , wherein the cell culture matrix is a sponge.
21 . The method of claim 20 , wherein the sponge is comprised of an alginate.
22 . A method of determining an effect of at least one compound on a cell comprising:
(a) coating a first surface with a positively charged molecule;
(b) contacting the first surface with a second surface, wherein a cell culture matrix comprises the second surface;
(c) contacting the cells with the cell culture matrix under conditions suitable for culturing the cells;
(d) contacting the cells of (c) with the at least one compound; and
(e) determining or detecting the effect or lack of effect of the at least one compound on the cell.
23 . The method of claim 22 , wherein a tissue culture vessel comprises the first surface.
24 . The method of claim 23 , wherein the coating of the first surface comprises contacting the first surface with a positively charged molecule in a solvent.
25 . The method of claim 24 , wherein the positively charged molecule is a polyamine.
26 . The method of claim 25 , wherein the polyamine is a polyallylamine.
27 . The method of claim 26 , wherein the cell culture matrix is a sponge.
28 . The method of claim 27 , wherein the sponge is comprised of an alginate.
29 . A method of producing a cell culture matrix, the method comprising contacting a first surface with the cell culture matrix, wherein the cell culture matrix comprises a positively charged molecule.
30 . A method of culturing cells on a cell culture matrix, the method comprising:
(a) contacting a first surface with the cell culture matrix, wherein the cell culture matrix comprises a positively charged molecule; and
(b) contacting the cells with the cell culture matrix under conditions suitable for culturing the cells.
31 . A method of determining an effect of at least one compound on a cell comprising:
(a) contacting a first surface with a cell culture matrix, wherein the cell culture matrix comprises a positively charged molecule;
(b) contacting the cells with the cell culture matrix under conditions suitable for culturing the cells;
(c) contacting the cells of (b) with the at least one compound; and
(d) determining or detecting the effect or lack of effect of the at least one compound on the cell.
32 . A method of adhering a cell to a surface comprising:
(a) coating a surface with a polyallylamine; and
(b) contacting the surface with a cell.