Method for measuring analysis object, biosensor and measuring device
View Patent ↗A biosensor includes a cavity for holding a sample, an electrode system for measuring the sample concentration in the cavity, and a thermocouple for obtaining temperature information during measurement. A measurement device includes a reference temperature junction sensor or an environmental temperature sensor for measuring the temperature of reference temperature junctions of the thermocouple. A temperature compensation measures the temperature difference between a measuring junction and a reference temperature junctions of the thermocouple, and specifies the temperature of a reference temperature junction, thereby using the temperature information as the temperature of a measurement component and performing accurate temperature compensation from the measuring junction temperature information and the environmental temperature information with respect to the measured values.
1. A biosensor, comprising:
a holder for holding an analysis object;
an electrode system for measuring the analysis object held by the holder; and
a thermocouple for forming a measuring junction by joining at least two dissimilar substances, and measuring a temperature of the analysis object.
2. The biosensor according to claim 1 ,
the measuring junction is disposed inside the holder.
3. The biosensor according to claim 1 ,
the measuring junction is disposed near the holder.
4. The biosensor according to claim 2 ,
wherein the measuring junction is disposed upstream from the electrode system with respect to a flow of a placed drop of the analysis object.
5. The biosensor according to claim 2 ,
wherein both ends that are part of the thermocouple are in locations that come into contact with thermocouple-use connection terminals provided to a measurement device, which measures a drop of the analysis object placed in the holder, when mounting to the measurement device.
6. The biosensor according to claim 1 ,
wherein the substance that makes up the thermocouple is composed of either a metal, an alloy, or a semiconductor, or a combination of these.
7. The biosensor according to claim 1 ,
wherein the holder has a cavity structure, and is formed by affixing a first substrate, a second substrate, and a third substrate together so that the analysis object will be supplied by capillary action.
8. The biosensor according to claim 1 ,
wherein the holder is formed by affixing two substrates together by the three-dimensional molding of the substrates.
9. The biosensor according to claim 1 ,
wherein the electrode system and the thermocouple are both provided on the same substrate.
10. The biosensor according to claim 1 ,
wherein the electrode system has its electrodes formed on mutually opposing substrates, with one electrode system and the thermocouple both formed on the same substrate.
11. The biosensor according to claim 1 ,
wherein the electrode system and the thermocouple are formed on mutually opposing substrates or substrate side faces.
12. The biosensor according to claim 1 ,
wherein one of the substances that form the thermocouple utilizes the wiring of the electrode system, or is made of the same material as the electrode system.
13. The biosensor according to claim 9 ,
wherein the thermocouple and the electrode system are formed on the substrates by sputtering, vapor deposition, or printing.
14. The biosensor according to claim 9 ,
wherein the electrode system is such that an electroconductive layer is formed over all or part of at least one face among the first substrate, the second substrate, and the third substrate, and slits are provided to form an electrode pattern.
15. The biosensor according to claim 9 ,
wherein the electrode system is such that an electrode pattern is formed by masking over all or part of at least one face among the first substrate, the second substrate, and the third substrate.
16. The biosensor according to claim 14 ,
wherein the thermocouple is such that a pattern is formed by masking over all or part of at least one face among the first substrate, the second substrate, and the third substrate.
17. The biosensor according to claim 1 ,
wherein all or part of the surface of the holder is covered with a surfactant in the substrate.
18. The biosensor according to claim 1 ,
wherein the electrode system has at least a working electrode and a counter electrode.
19. The biosensor according to claim 18 ,
wherein a measurement reagent for oxidizing or reducing a specific target component is provided to the holder.
20. The biosensor according to claim 1 ,
which is used disposably.