A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrile hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrile wherein the nitrile is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NCIMB 41164, a mutant thereof and a nitrile hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NCIMB 41164.
1. A process of preparing an amide from the corresponding nitrile comprising:
subjecting the nitrile to a hydration reaction in an aqueous medium in the presence of a biocatalyst wherein the biocatalyst is Rhodococcus rhodochrous strain NCIMB 41164 microorganism.
2. A process according to claim 1 in which the amide is (meth)acrylamide.
3. A process according to claim 1 in which the biocatalyst is introduced into an aqueous medium and (meth)acrylonitrile is fed into the aqueous medium such that the concentration of (meth)acrylonitrile in the aqueous medium is maintained at up to 6% by weight.
4. A process according to claim 3 in which the reaction continues until the concentration of acrylamide is between 30 and 55% by weight.
5. A process according to claim 1 in which the biocatalyst is recycled.
6. A process of preparing an amide from the corresponding nitrile comprising:
subjecting the nitrile to a hydration reaction in an aqueous medium in the presence of Rhodococcus rhodochrous strain NCIMB 41164 microorganism which has been cultured in a culture medium that comprises urea or a derivative of urea, wherein urea or the derivative of urea is introduced into the culture medium at least 6 hours after the start of growth of the microorganism.
7. A process according to claim 6 in which the amide is (meth)acrylamide.
8. A process according to claim 6 in which the biocatalyst is introduced into an aqueous medium and (meth)acrylonitrile is fed into the aqueous medium such that the concentration of (meth)acrylonitrile in the aqueous medium is maintained at up to 6% by weight.
9. A process according to claim 8 in which the reaction continues until the concentration of acrylamide is between 30 and 55% by weight.
10. A process according to claim 6 in which the biocatalyst is recycled.