Transaminase polypeptides
View Patent ↗The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
1. An engineered transaminase enzyme, comprising:
a polypeptide comprising an amino acid sequence that has a T residue at position X9 and is at least 90% identical to SEQ ID NO: 6, wherein the polypeptide has transaminase activity.
2. The engineered transaminase enzyme of claim 1 , wherein the polypeptide has at least 10% residual activity in the conversion of pyruvate to L-alanine in presence of amino donor isopropylamine after treatment of the polypeptide at 50° C. for 23 h.
3. The engineered transaminase enzyme of claim 1 , wherein the amino acid sequence further comprises one or more of the amino acid substitutions selected from the group consisting of: X45 is H; X86 is Y, F, S, N, A, G, or H; X177 is L; X211 is K; X294 is V; X324 is G; and X391 is A.
4. The engineered transaminase enzyme of claim 3 , wherein the amino acid sequence comprises the amino acid substitutions: X45 is H, X86 is Y, X177 is L, X211 is K, X294 is V, X324 is G, and X391 is A.
5. The engineered transaminase enzyme of claim 3 , wherein the enzyme further comprises increased transaminase activity for conversion of an amino acceptor substrate to the corresponding chiral amino product as compared to the enzyme of SEQ ID NO: 2.
6. The engineered transaminase enzyme of claim 1 , which comprises an amino acid sequence corresponding to SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, or 192.
7. A method for the conversion of a substrate of Formula I to a chiral amine product of Formula III in stereomeric excess, the method comprising contacting the substrate of Formula I with the engineered transaminase polypeptide of claim 1 in the presence of an amino donor under suitable reaction conditions for the conversion of the substrate of Formula Ito the chiral amine product of Formula III
wherein
the chiral carbon atom of the chiral amine product is marked with an *;
each of R 1 , and R 2 , when taken independently, is an unsubstituted or substituted alkyl, alkylaryl, or aryl group, wherein R 1 is different from R 2 in structure or chirality, or R 1 and R 2 , taken together, is a hydrocarbon chain of 4 or more carbon atoms containing a center of chirality.