System for processing cells and container for use therewith
View Patent ↗A system for processing cells is disclosed. The system may include a container having an inner wall, a plunger adapted to be slidably positioned within the container, at least one inlet port through which a fluid can enter the container, at least one outlet port through which a fluid can exit the container, at least one effluent port through which an effluent can exit the container, a first check valve in fluid connection with the inlet port and a second check valve in fluid connection with the effluent port. The plunger may include a filter that allows fluid to pass therethrough but prevents cells from passing therethrough. Rearward motion of the plunger may be adapted to draw fluid into the system via the inlet port and forward motion of the plunger may be adapted to force effluent out of the system via the effluent port.
1. A system for processing cells, comprising:
a container having an inner wall;
a plunger adapted to be slidably positioned within the container, the plunger comprising a filter that allows fluid to pass therethrough but prevents cells from passing therethrough;
at least one inlet port through which the fluid can enter the container;
at least one outlet port through which the fluid can exit the container;
at least one effluent port through which an effluent can exit the container;
a first check valve in fluid connection with the inlet port; and
a second check valve in fluid connection with the effluent port, wherein the inlet port and the effluent port are positioned on one side of the plunger, the outlet port is positioned on an opposite side of the plunger, and the plunger forms a sealing engagement with the inner wall of the container such that rearward motion of the plunger is adapted to draw the fluid into the system via the inlet port and forward motion of the plunger is adapted to force the effluent out of the system via the effluent port.
2. The system of claim 1 wherein the effluent port is adapted to effect delivery of the cells therethrough to a patient.
3. The system of claim 1 wherein the outlet port adapted to effect delivery of the cells therethrough to a patient.
4. The system of claim 1 wherein the filter is in fluid connection with the effluent port to prevent cells from exiting via the effluent port.
5. The system of claim 1 wherein the filter separates the cells from the effluent port.
6. The system of claim 5 wherein the filter also separates the cells from the inlet port.
7. The system of claim 6 wherein the outlet port is adapted to effect delivery of cells therethrough to a patient.
8. The system of claim 7 wherein the outlet port is further adapted to be closed during processing of the cells during which the fluid enters the system via the inlet port and the effluent exits the system via the effluent port.
9. The system of claim 8 wherein the effluent port is adapted to be closed when the outlet port is opened to deliver the cells therethrough.
10. The system of claim 7 wherein the inlet port is in fluid connection with a passage through the plunger so that the fluid can enter the plunger and pass through the filter.
11. The system of claim 7 wherein the effluent port is in fluid connection with a passage through the plunger so that the effluent can pass through the filter and exit the effluent port.
12. The system of claim 1 wherein the plunger includes a sealing member adapted to form the sealing engagement with the interior wall of the container.