Analysis device, and analysis apparatus and method using the same

1. A fluid handling device having a microchannel structure that transfers a sample liquid towards a measurement spot by a centrifugal force about a rotation axis, the fluid handling device being used for reading involving accessing a reaction liquid at the measurement spot, the fluid handling device comprising:

a fluid handling device main body having, formed inside, a microchannel structure with minute surface irregularities;

a protective cap that exposes, in an open position, an inlet for collecting the sample liquid into the microchannel structure, and in a closed position, that covers a part of the fluid handling device main body, thereby preventing the inlet from being exposed;

a diluent container whose opening is sealed by a seal member so as to internally hold a diluent;

a diluent container containing section inside the fluid handling device main body, the diluent container containing section holding the diluent container such that the diluent container is slidably movable in a transverse direction relative to the rotation axis between a liquid holding position and a liquid discharge position;

a protrusion provided so as to protrude along a movement path of the diluent container from the liquid holding position to the liquid discharge position in the diluent container containing section, the protrusion breaking the seal member of the diluent container when the diluent container moves to the liquid discharge position so as to open the diluent container; a latch positioned at a side of the dilute container facing the protective cap; and

a locking groove formed on the protective cap, the locking groove being engaged to the latch when the diluent container is positioned in the liquid holding position so as to prevent the diluent container from moving toward the liquid discharge position,

wherein the latch and the locking groove are disengaged by a shifting of the protective cap from the closed position to the open position, and

wherein a wall face facing the diluent container is formed on the protective cap next to the locking groove, when the protective cap shifts from the open position to the closed position, the wall face abuts a face of the latch that faces the protective cap and pushes the diluent container from the liquid holding position to the liquid discharge position, thereby allowing the protrusion to collide with the seal member and break the seal member.

2. The fluid handling device according to claim 1 , wherein

a seal face on which the seal member of the diluent container is to be attached is formed inclined.

3. A fluid handling method utilizing the fluid handling device according to claim 1 ,

the fluid handling method comprising:

spot-applying the sample liquid onto the inlet exposed by opening the protective cap of the fluid handling device and collecting the sample liquid;

pushing the diluent container positioned in the diluent container containing section of the fluid handling device towards the protrusion by an operation of the protective cap from the open position to the closed position;

pressing the seal member of the diluent container against the protrusion so as to break the seal member and open the diluent container; and

setting the fluid handling device onto a rotor having a rotation axis and rotating the rotor so as to dilute at least a portion of the sample liquid spot-applied to the fluid handling device by the diluent discharged from the diluent container.

4. The fluid handling device according to claim 1 , wherein the latch is integral with the dilute container.