Functionalized polysaccharides for active agent delivery
Embodiments of the invention include functionalized polysaccharides and compositions and structures including the same. In an embodiment, the invention includes an active agent delivery composition including a polysaccharide functionalized with a coupling group, wherein the polysaccharide lacks charged groups at a pH of between 6 and 8; and a complex comprising a nucleic acid and a transfection agent. In an embodiment, the invention includes an active agent delivery structure including a matrix comprising a polysaccharide covalently cross-linked through the residue of a coupling group on the polysaccharide, the polysaccharide lacking charged groups at a pH of between 6 and 8; and a nucleic acid delivery complex disposed within the active agent delivery structure. In an embodiment, the invention includes a material for medical applications including glycogen functionalized with coupling groups at a degree of substitution of between about 0.01 and 0.5. Other embodiments are also included herein.
1. An active agent delivery composition comprising
a polysaccharide functionalized with a coupling group, wherein the polysaccharide lacks charged groups at a pH of between 6 and 8, wherein the degree of substitution is greater than or equal to 0.01 and less than or equal to 0.2; and
a complex comprising a nucleic acid and a transfection agent.
2. The active agent delivery composition of claim 1 , the polysaccharide comprising a branched polysaccharide.
3. The active agent delivery composition of claim 1 , the polysaccharide comprising alpha 1,6 glycosidic linkages.
4. The active agent delivery composition of claim 1 , further comprising a protein core, the polysaccharide contacting the protein core.
5. The active agent delivery composition of claim 1 , the polysaccharide comprising glycogen or derivative thereof.
6. The active agent delivery composition of claim 1 , the coupling group comprising an ethylenically unsaturated group.
7. The active agent delivery composition of claim 1 , the coupling group comprising a methacrylate group.
8. The active agent delivery composition of claim 1 , the nucleic acid comprising siRNA.
9. The active agent delivery composition of claim 1 , the transfection agent comprising a cationic lipid.
10. The active agent delivery composition of claim 1 , further comprising a crosslinking agent.
11. An active agent delivery structure comprising
a matrix comprising a polysaccharide covalently cross-linked through the residue of a coupling group on the polysaccharide, the polysaccharide lacking charged groups at a pH of between 6 and 8, wherein the degree of substitution is greater than or equal to 0.01 and less than or equal to 0.2; and
a nucleic acid delivery complex disposed within the active agent delivery structure, the nucleic acid delivery complex comprising a nucleic acid and a transfection agent.
12. The active agent delivery structure of claim 11 , the polysaccharide comprising a branched polysaccharide.
13. The active agent delivery structure of claim 11 , the polysaccharide comprising alpha 1,6 glycosidic linkages.
14. The active agent delivery structure of claim 11 , further comprising a protein core, the polysaccharide contacting the protein core.
15. The active agent delivery structure of claim 11 , the polysaccharide comprising glycogen or derivative thereof.
16. The active agent delivery structure of claim 11 , the coupling group comprising an ethylenically unsaturated group.
17. The active agent delivery structure of claim 11 , the coupling group comprising a methacrylate group.
18. The active agent delivery structure of claim 11 , the nucleic acid comprising siRNA.
19. The active agent delivery structure of claim 11 , further comprising a crosslinking agent.
20. The active agent delivery composition of claim 1 , wherein the degree of substitution is less than or equal to 0.15.