Compositions and methods for recruiting and localizing stem cells
Described herein are compositions and methods of using modified placental tissue to achieve endogenous and exogenous therapeutic effects. When applied to an injured or diseased organ or body part, the modified placental tissue elicit stem cell recruitment and/or localization directly to or proximate to the site of application. Also described is a novel vacuum drying device and the use thereof.
1. A method of eliciting stem cell recruitment and/or localization to a diseased or injured body part, the method comprising contacting a source of stem cells with a sufficient amount of modified placental tissue under conditions such that the placental tissue elutes an effective amount of stem cell recruiting factors so as to promote sufficient stem cell recruitment and/or localization proximate to said placental tissue to treat the diseased or injured body part.
2. The method of claim 1 , wherein the source is ex vivo.
3. The method of claim 1 , wherein the stem cells recruited by the composition are pluripotent stem cells.
4. The method of claim 1 , wherein the placental tissue has a mass sufficient to recruit stem cells.
5. The method of claim 1 , wherein the placental tissue comprises amnion.
6. The method of claim 5 , wherein the amnion is selected from the group consisting of decellularized amnion, deepithelialized amnion retaining a fibroblast layer and amnion containing both epithelial cells and a fibroblast layer.
7. The method of claim 1 , wherein the stem cell recruitment and/or localization occurs within about one day to at least 7 days after contacting with the placental tissue.