Granted Patent
B2
US 9,273,366 · App. 14/119,563 · Granted Mar 1, 2016
Methods, compositions, and kits for determining human immunodeficiency virus (HIV)
Inventors:
Danuta Wronska (Raleigh, NC); Katherine Schouest (Cary, NC); Leslie Tremlett (Raleigh, NC)
Assignee:
Grifols Therapeutics Inc.
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Abstract
The present invention relates to compositions, methods, and kits for determining the presence or absence of HIV in a sample, in particular for determining HIV-1 group M, HIV-1 group O, and/or HIV-2, in particular for simultaneous determining of HIV-1 group M, HIV-1 group O, and HIV-2.
Claims (12)
1. A composition comprising:
(a) an oligonucleotide comprising the sequence of SEQ ID NO:10; and
(b) a first forward primer comprising the sequence of SEQ ID NO:14 and a first reverse primer comprising the sequence of SEQ ID NO:15, wherein the first forward primer and the first reverse primer each is capable of annealing to a target sequence under a PCR condition to thereby amplify the target sequence.
2. The composition of claim 1 , further comprising an oligonucleotide probe comprising the sequence of SEQ ID NO:6, a second forward primer comprising the sequence of SEQ ID NO:16 and a second reverse primer comprising the sequence of SEQ ID NO:17.
3. A kit comprising the composition of claim 2 .
4. A method for amplifying a target sequence, comprising:
(a) contacting the composition of claim 1 with the target sequence; and
(b) performing a PCR using the target sequence as template, the forward primer and the reverse primer, whereby the target sequence is amplified.
5. A kit comprising the composition of claim 1 .
6. A method for determining human immunodeficiency virus (HIV) in a sample, comprising:
(a) performing a PCR with a nucleic acid template in the sample using a forward primer comprising the sequence of SEQ ID NO:14 and a reverse primer comprising the sequence of SEQ ID NO:15, whereby an amplicon is generated; and
(b) detecting the amplicon with an oligonucleotide comprising the sequence of SEQ ID NO:10, wherein the detection of the amplicon is indicative of the presence of HIV in the sample.
Assignments (28)
RELEASE OF SECURITY INTEREST IN PATENTS, RECORDED ON DECEMBER 10, 2021 AT REEL 058480 FRAME 0221
Recorded May 21, 2026
From: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (FORMERLY KNOWN AS GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (FORMERLY KNOWN AS GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS USA, LLC; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 075614/0936 →
RELEASE OF SECURITY INTEREST
Recorded Apr 15, 2026
From: EUROPEAN INVESTMENT BANK
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (F/K/A GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (F/K/A GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 075416/0633 →
RELEASE OF SECURITY INTEREST
Recorded Apr 15, 2026
From: EUROPEAN INVESTMENT BANK
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (F/K/A GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (F/K/A GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 075416/0698 →
RELEASE OF SECURITY INTEREST
Recorded Apr 15, 2026
From: EUROPEAN INVESTMENT BANK
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (F/K/A GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (F/K/A GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 075416/0718 →
RELEASE OF SECURITY INTEREST
Recorded Apr 15, 2026
From: THE BANK OF NEW YORK MELLON, LONDON BRANCH
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (F/K/A GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (F/K/A GRIFOLS BIOLOGICALS, INC.); GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; BIOMAT USA, INC.; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 075329/0824 →
SECURITY INTEREST
Recorded May 14, 2024
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC; GRIFOLS BIOLOGICALS LLC; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC
To: THE BANK OF NEW YORK MELLON, LONDON BRANCH
Reel/Frame 067409/0127 →
PARTIAL RELEASE OF SECURITY INTEREST
Recorded Dec 10, 2021
From: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
To: BIOMAT USA, INC.; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 058480/0221 →
PARTIAL RELEASE OF SECURITY INTEREST IN PATENTS AT REEL/FRAME NO. 51024/0317
Recorded Dec 7, 2021
From: EUROPEAN INVESTMENT BANK
To: BIOMAT USA, INC.; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 058518/0524 →
PARTIAL RELEASE OF SECURITY INTEREST IN PATENTS AT REEL/FRAME NO. 51024/0453
Recorded Dec 7, 2021
From: EUROPEAN INVESTMENT BANK
To: BIOMAT USA, INC.; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 058351/0064 →
PARTIAL RELEASE OF SECURITY INTEREST IN PATENTS AT REEL/FRAME NO. 51021/0593
Recorded Dec 7, 2021
From: EUROPEAN INVESTMENT BANK
To: BIOMAT USA, INC.; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 058350/0980 →
PARTIAL RELEASE OF SECURITY INTEREST IN PATENTS AT REEL/FRAME NO. 51047/0139
Recorded Dec 1, 2021
From: THE BANK OF NEW YORK MELLON, LONDON BRANCH
To: BIOMAT USA, INC.; TALECRIS PLASMA RESOURCES, INC.
Reel/Frame 058292/0564 →
SECURITY INTEREST
Recorded Nov 18, 2019
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC; GRIFOLS BIOLOGICALS LLC; BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
To: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
Reel/Frame 051047/0123 →
SECURITY INTEREST
Recorded Nov 18, 2019
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC; GRIFOLS BIOLOGICALS LLC; BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
To: THE BANK OF NEW YORK MELLON, LONDON BRANCH, AS NOTES COLLATERAL AGENT
Reel/Frame 051047/0139 →
RELEASE OF SECURITY INTEREST
Recorded Nov 18, 2019
From: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (FORMERLY KNOWN AS GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (FORMERLY KNOWN AS GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS DIAGNOSTIC SOLUTIONS INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC
Reel/Frame 051047/0153 →
SECURITY INTEREST
Recorded Nov 15, 2019
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC; GRIFOLS BIOLOGICALS LLC; BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
To: EUROPEAN INVESTMENT BANK
Reel/Frame 051024/0317 →
SECURITY INTEREST
Recorded Nov 15, 2019
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC; GRIFOLS BIOLOGICALS LLC; BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
To: EUROPEAN INVESTMENT BANK
Reel/Frame 051021/0593 →
SECURITY INTEREST
Recorded Nov 15, 2019
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC; GRIFOLS BIOLOGICALS LLC; BIOMAT USA, INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC; TALECRIS PLASMA RESOURCES, INC.
To: EUROPEAN INVESTMENT BANK
Reel/Frame 051024/0453 →
RELEASE OF SECURITY INTEREST
Recorded Nov 15, 2019
From: EUROPEAN INVESTMENT BANK
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (FORMERLY KNOWN AS GRIFOLS THERAPEUTICS, INC.); GRIFOLS BIOLOGICALS LLC (FORMERLY KNOWN AS GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS DIAGNOSTIC SOLUTIONS INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.
Reel/Frame 051024/0722 →
RELEASE OF SECURITY INTEREST
Recorded Nov 15, 2019
From: EUROPEAN INVESTMENT BANK
To: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC (FORMERLY KNOWN AS GRIFOLS THERAPEUTICS, INC.; GRIFOLS BIOLOGICALS LLC (FORMERLY KNOWN AS GRIFOLS BIOLOGICALS, INC.); BIOMAT USA, INC.; GRIFOLS DIAGNOSTIC SOLUTIONS INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC
Reel/Frame 051064/0021 →
SECURITY INTEREST
Recorded Sep 7, 2018
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS LLC; GRIFOLS BIOLOGICALS LLC; BIOMAT USA, INC.; GRIFOLS DIAGNOSTIC SOLUTIONS INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC
To: EUROPEAN INVESTMENT BANK, AS LENDER
Reel/Frame 047438/0836 →
CHANGE OF ADDRESS
Recorded Jun 1, 2018
From: GRIFOLS THERAPEUTICS LLC
To: GRIFOLS THERAPEUTICS LLC
Reel/Frame 046286/0824 →
CHANGE OF NAME
Recorded Jan 5, 2018
From: GRIFOLS THERAPEUTICS INC.
To: GRIFOLS THERAPEUTICS LLC
Reel/Frame 045008/0573 →
SECURITY INTEREST
Recorded Dec 5, 2017
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS INC.; GRIFOLS BIOLOGICALS INC.; BIOMAT USA, INC.; GRIFOLS DIAGNOSTIC SOLUTIONS.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC
To: EUROPEAN INVESTMENT BANK, AS LENDER
Reel/Frame 044697/0725 →
SECURITY INTEREST
Recorded Dec 5, 2017
From: GRIFOLS SHARED SERVICES NORTH AMERICA, INC.; GRIFOLS THERAPEUTICS INC.; GRIFOLS BIOLOGICALS INC.; BIOMAT USA, INC.; GRIFOLS DIAGNOSTIC SOLUTIONS.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.
To: EUROPEAN INVESTMENT BANK, AS LENDER
Reel/Frame 044696/0846 →
SECURITY INTEREST
Recorded Feb 7, 2017
From: GRIFOLS SHARED SERVICES NORTH AMERICA INC.; GRIFOLS THERAPEUTICS INC.; GRIFOLS BIOLOGICALS INC.; BIOMAT USA, INC.; GRIFOLS DIAGNOSTIC SOLUTIONS INC.; GRIFOLS WORLDWIDE OPERATIONS USA, INC.; GRIFOLS USA, LLC
To: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
Reel/Frame 041651/0487 →
RELEASE OF SECURITY INTEREST
Recorded Feb 6, 2017
From: DEUTSCHE BANK AG NEW YORK BRANK
To: GRIFOLS THERAPEUTICS INC.; GRIFOLS SHARED SERVICES NORTH AMERICA INC.; GRIFOLS DIAGNOSTIC SOLUTIONS INC.
Reel/Frame 041638/0527 →
SECURITY AGREEMENT
Recorded Feb 27, 2014
From: GRIFOLS INC.; GRIFOLS THERAPEUTICS INC.; GRIFOLS-CHIRON DIAGNOSTICS CORP.
To: DEUTSCHE BANK AG NEW YORK BRANCH, AS COLLATERAL AGENT
Reel/Frame 032367/0001 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 22, 2013
From: WRONSKA, DANUTA; SCHOUEST, KATHERINE; TREMLETT, LESLIE
To: GRIFOLS THERAPEUTICS INC.
Reel/Frame 031660/0489 →
Continuity (2)
Provisional Application
61497234
· Jun 15, 2011