Biosensor, biosensor chip and biosensor device
View Patent ↗A biosensor includes a working electrode 101 , a counter electrode 102 opposing the working electrode 101 , a working electrode terminal 103 and a working electrode reference terminal 10 connected to the working electrode 101 by wires, and a counter electrode terminal 104 connected to the counter electrode 102 by a wire. By employing a structure with at least three electrodes, it is possible to assay a target substance without being influenced by the line resistance on the working electrode side.
1. A biosensor, comprising:
a working electrode disposed on a proximal end of the biosensor;
a counter electrode disposed on the proximal end of the biosensor;
a working electrode terminal on a distal end of the biosensor;
a counter electrode terminal on the distal end of the biosensor;
a first reference terminal on the distal end of the biosensor;
a second reference terminal on the distal end of the biosensor;
a first conductive line connecting the working electrode to the working electrode terminal;
a second conductive line connecting the working electrode to the first reference terminal, at least a portion of the second conductive line disposed outside at least a portion of the first conductive line relative to a centerline that extends between the proximal end and the distal end of the biosensor;
a third conductive line connecting the counter electrode to the counter electrode terminal; and
a fourth conductive line connecting the counter electrode to the second reference terminal, at least a portion of the fourth conductive line disposed outside at least a portion of the third conductive line relative to the centerline.
2. The biosensor of claim 1 , wherein:
the second conductive line is provided to enable a voltage at the working electrode to be detected; and
the fourth conductive line is provided to enable a voltage at the counter electrode to be detected.
3. The biosensor of claim 1 , wherein the second conductive line and the fourth conductive line are provided to enable a voltage between the working electrode and the counter electrode to be constant.
4. The biosensor of claim 1 , wherein at least a portion of the first conductive line is disposed between at least a portion of the second conductive line and at least a portion of the third conductive line on the biosensor.
5. The biosensor of claim 1 , wherein at least a portion of the third conductive line is disposed between at least a portion of the first conductive line and at least a portion of the fourth conductive line on the biosensor.
6. The biosensor of claim 1 , wherein:
a portion of the first conductive line and the second conductive line is partially shared; and
the partially shared portion is connected to the working electrode.
7. The biosensor of claim 1 , wherein:
a portion of the third conductive line and the fourth conductive line is partially shared; and
the shared portion is connected to the counter electrode.
8. The biosensor of claim 1 , wherein the counter electrode comprises two fingers.
9. The biosensor of claim 1 , wherein:
the counter electrode comprises two fingers; and
the working electrode is provided between the two fingers.
10. The biosensor of claim 1 , wherein the first reference terminal is disposed near an outside edge of the biosensor relative to the centerline.
11. The biosensor of claim 1 , wherein the second reference terminal is disposed near an outside edge of the biosensor relative to the centerline.
12. The biosensor of claim 1 , further comprising an assay reagent applied on the working electrode and on the counter electrode.
13. A biosensor device, comprising:
a biosensor comprising:
a working electrode disposed on a proximal end of the biosensor;
a counter electrode disposed on the proximal end of the biosensor;
a working electrode terminal on a distal end of the biosensor;
a counter electrode terminal on the distal end of the biosensor;
a first reference terminal on the distal end of the biosensor;
a second reference terminal on the distal end of the biosensor;
a first conductive line connecting the working electrode to the working electrode terminal;
a second conductive line connecting the working electrode to the first reference terminal, at least a portion of the second conductive line disposed outside at least a portion of the first conductive line relative to a centerline that extends between the proximal end and the distal end of the biosensor;
a third conductive line connecting the counter electrode to the counter electrode terminal; and
a fourth conductive line connecting the counter electrode to the second reference terminal, at least a portion of the fourth conductive line disposed outside at least a portion of the third conductive line relative to the centerline; and
a measurement circuit configured to be connected to the biosensor for performing an assay of a fluid to determine the concentration of a substance in the fluid based on a current flowing between the working electrode and the counter electrode of the biosensor.
14. The biosensor device of claim 13 , wherein substantially no current flows through the second conductive line and the fourth conductive line during the assay.
15. The biosensor device of claim 13 , wherein:
the second conductive line is provided to enable a voltage at the working electrode to be detected; and
the fourth conductive line is provided to enable a voltage at the counter electrode to be detected.
16. The biosensor device of claim 13 , wherein the second conductive line and the fourth conductive line are provided to enable a voltage between the working electrode and the counter electrode to be constant.
17. The biosensor device of claim 13 , wherein at least a portion of the first conductive line is disposed between at least a portion of the second conductive line and at least a portion of the third conductive line on the biosensor.
18. The biosensor device of claim 13 , wherein at least a portion of the third conductive line is disposed between at least a portion of the first conductive line and at least a portion of the fourth conductive line on the biosensor.
19. The biosensor device of claim 13 , wherein:
a portion of the first conductive line and the second conductive line is partially shared; and
the shared portion is connected to the working electrode.
20. The biosensor device of claim 13 , wherein:
a portion of the third conductive line and the fourth conductive line is partially shared; and
the shared portion is connected to the counter electrode.
21. The biosensor device of claim 13 , wherein the counter electrode comprises two fingers.
22. The biosensor device of claim 13 , wherein:
the counter electrode comprises two fingers; and
the working electrode is provided between the two fingers.
23. The biosensor device of claim 13 , wherein the first reference terminal is disposed near an outside edge of the biosensor relative to the centerline.
24. The biosensor of claim 13 , wherein the second reference terminal is disposed near an outside edge of the biosensor relative to the centerline.
25. The biosensor device of claim 13 , further comprising an assay reagent applied on the working electrode and on the counter electrode.