Collagen reinforced tissue grafts
This invention is directed to a multi-layered tissue graft comprising a collagen layer and at least one separated and washed placental tissue component and/or umbilical cord component, wherein the collagen is human collagen substantially free of non-human antigens.
1. A multi-layered tissue graft comprising a collagen layer and at least one separated and washed placental tissue layer derived from at least one amnion layer, wherein the at least one separated and washed placental tissue layer comprises a basement membrane and a compact layer, and wherein the collagen layer is reconstituted human collagen substantially free of immunogenic agents.
2. The multi-layered tissue graft of claim 1 , comprising a collagen layer interposed between the at least one separated and washed placental tissue layer and a second separated and washed placental tissue layer and/or umbilical cord component.
3. The multi-layered tissue graft of claim 1 , wherein the collagen layer is at least 10 wt. % relative to the total weight % of the tissue graft.
4. The multi-layered tissue graft of claim 1 , wherein the layers are laminated together.
5. The multi-layered tissue graft of claim 1 , wherein the collagen layer is crosslinked.
6. The multi-layered tissue graft of claim 1 , wherein the collagen layer has a tensile strength of at least about 100 kilopascals.
7. The multi-layered tissue graft of claim 6 , wherein the collagen layer has a tensile strength of between about 500 kilopascals to about 10 megapascals.
8. The multi-layered tissue graft of claim 1 , wherein the collagen layer has a stiffness of at least about 50 megapascals.
9. The multi-layered tissue graft of claim 8 , wherein the collagen layer has a stiffness of between about 100 megapascals and about 500 megapascals.