METHODS AND COMPOSITIONS FOR USING SOLVATOCHROMIC DYES TO DETECT THE PRESENCE OF HOMEOPATHIC POTENCIES
A method is provided for obtaining a characteristic of a homeopathic preparation, particularly its potency. In particular aspects, solvatochromic dyes may be used for detection of a homeopathic preparation.
1 . A method of quantifying a homeopathic potency effect, comprising the steps of:
a) obtaining a sample solution by adding a homeopathic potency solution to a dye solution comprising a polar and polarizable molecule in a solvent;
b) obtaining a reference solution comprising an equivalent amount of the dye solution of step a);
c) obtaining a spectrum of the sample solution;
d) obtaining a spectrum of the reference solution;
e) obtaining a difference spectrum, wherein the difference spectrum is the difference between the sample solution spectrum and the reference solution spectrum; and
f) quantifying the potency effect of the homeopathic potency solution by identifying a maximum value of the difference spectrum.
2 . The method of claim 1 , wherein the homeopathic potency solution has been prepared by a serial dilution of between 100 6 to 100 100000 .
3 . The method of claim 2 , wherein the homeopathic potency solution has been prepared by a serial dilution of more than 100 200 .
4 . The method of claim 2 , wherein the homeopathic potency solution has been prepared by a serial dilution of up to 100 50,000 .
5 . The method of any of claims 1 - 4 , wherein the polar and polarizabale molecule is a negatively solvatochromic dye.
6 . The method of any of claims 1 - 4 , wherein the polar and polarizabale molecule is a positively solvatochromic dye.
7 . The method of claim 5 , wherein the negatively solvatochromic dye is ET33, ET30, or BM.
8 . The method of claim 6 , wherein the positively solvatochromic dye is BDN, BDF, NR, or PB.
9 . The method of any of claims 1 - 4 , wherein the polar and polarizable molecule is a halochromic dye.
10 . The method of claim 8 . 1 , wherein the halochromic dye is 6-amino-2-naphthoic acid (6-ANA) or 6-amino-2-anthracenic acid (6-AAA).
11 . The method of any of claims 1 - 8 , wherein the solvent is an ionic solvent or a non-ionic solvent.
12 . The method of any of claims 1 - 9 , wherein the dye solution has a polar and polarizable molecule concentration of from 10 μM to 250 μM.
13 . The method of any of claims 1 - 10 , wherein the dye solution has a polar and polarizable molecule concentration that gives absorbance of c. 1.0 at its absorbance maxima.
14 . The method of any of claims 1 - 11 , wherein the reference solution spectrum is a control.
15 . The method of any of claims 1 - 12 , wherein the measuring step comprises obtaining a plurality of sample solution and reference solution spectra at time intervals for up to 20 days.
16 . The method of any of claims 1 - 12 , wherein the measuring step comprises obtaining a plurality of sample solution and reference solution spectra at time intervals for up to 24 hours.
17 . The method of any of claims 1 - 14 , wherein the time interval may range from one minute to one hour.
18 . The method of any of claims 1 - 15 , wherein the step of obtaining a spectrum comprises measuring an absorbance spectrum.
19 . The method of any of claims 1 - 16 , wherein the step of obtaining a spectrum comprises measuring a fluorescence spectrum.
20 . The method of any of claims 1 - 17 , wherein the sample solution has a volume of about 300 μL to 3 mL.
21 . The method of any of claims 1 - 16 , wherein the measuring comprises the use of a spectrophotometer.
22 . The method of any of claims 1 - 19 , wherein the sample solution is kept in a quartz or glass cuvette during measuring.
23 . The method of any of claims 1 - 20 , wherein the maximum value of the difference spectrum comprises the maximum of all difference spectra obtained over all time interval measurements.
24 . The method of any of claims 1 - 21 , wherein a plot of the maximum of each difference spectrum vs. time is employed to determine a maximum rate of change of difference spectra.
25 . The method of any of claims 1 - 6 or 9 - 22 , wherein the polar and polarizable molecule is a molecule having conjugated π-electron system through which negative charge may be delocalized.
26 . The method of any of claims 1 - 6 or 9 - 22 , wherein the polar and polarizable molecule is a molecule having electron donor and acceptor moieties linked through an electron delocalized system in which the compound's dipole moment in the electronic ground state is considerably different from that in the excited state.
27 . The method of any of claims 1 - 6 or 9 - 22 , wherein the polar and polarizable molecule is a dye selected from the group consisting of ET30, ET33, BM, BDF, BDN, PB, NR, DCM, PRODAN, DCVJ, phenol blue, a stilbazolium dye, a coumarin dye, a ketocyanine dye, analogs of Reichardt's dye, merocyanine dyes, or NDMNA.
28 . The method of any of claims 1 - 23 , wherein the solvent further comprises a buffer.
29 . The method of claim 24 , wherein the buffer is sodium borate/boric acid buffer, disodium citrate/trisodium citrate buffer, monopotassium phosphate/dipotassium phosphate buffer, Dimethylarsinic acid (cacodylic acid) buffer, veronal-acetate buffer, s-Collidine buffer, 3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid (TAPSO) buffer, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES) buffer, 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid (TES) buffer, 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer, 1,4-Piperazinediethanesulfonic acid (PIPES) buffer, or 2-(N-morpholino)ethanesulfonic acid (MES) buffer.
30 . The method of any of claims 1 - 25 , wherein the homeopathic potency solution, polar and polarizable molecule solution, reference solution, or sample solution are prepared in the absence of light.
31 . The method of any of claims 1 - 26 , wherein the homeopathic potency solution, polar and polarizable molecule solution, reference solution, or sample solution are kept in the absence of light from preparation time until a time of spectral determination.
32 . The method of any of claim 26 or 27 , wherein the light is light with a wavelength of greater than 350 nm.
33 . The method of any of claims 1 - 28 , wherein the homeopathic potency solution, polar and polarizable molecule solution, reference solution, or sample solution are incubated for a period of up to 20 days prior to a spectral determination thereof.
34 . The method of claim 9 , wherein the ionic solvent is 1-ethyl-3-methylimidazolium acetate.
35 . The method of claim 9 , wherein the non-ionic solvent is water, ethanol, tert-butyl alcohol, or a combination thereof.
36 . The method of any of claims 1 - 4 or 9 - 31 , wherein the polar and polarizable molecule comprises a compound of the structure
wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , and R 16 are each independently selected from hydrogen, alkyl, acyl, haloalky, hydroxyl, alkoxy, haloalkoxy, amino, nitro, mercapto, cyano, silyl, alkylsilyl, alkenyl, alkynyl, aryl, aralkyl, alkenoxy, alkynoxy, aryloxy, acyloxy, alkylamino, alkenylamino, alkynylamino, arylamino, amido, alkylthio, alkenylthio, alkynylthio, and arylthio.
37 . The method of any of claims 1 - 4 or 9 - 31 , wherein the polar and polarizable molecule comprises a compound of the structure
wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , and R 11 are each independently selected from hydrogen, alkyl, acyl, haloalky, hydroxyl, alkoxy, haloalkoxy, amino, nitro, mercapto, cyano, silyl, alkylsilyl, alkenyl, alkynyl, aryl, aralkyl, alkenoxy, alkynoxy, aryloxy, acyloxy, alkylamino, alkenylamino, alkynylamino, arylamino, amido, alkylthio, alkenylthio, alkynylthio, and arylthio.
38 . The method of any of claims 1 - 33 , wherein the maximum value of the difference spectrum correlates with a dipole moment of the polar and polarizable molecule.